Transcriptomic insights on the virulence-controlling CsrA, BadR, RpoN, and RpoS regulatory networks in the Lyme disease spirochete

PloS One
William K ArnoldBrian Stevenson

Abstract

Borrelia burgdorferi, the causative agent of Lyme disease, survives in nature through a cycle that alternates between ticks and vertebrates. To facilitate this defined lifestyle, B. burgdorferi has evolved a gene regulatory network that ensures transmission between those hosts, along with specific adaptations to niches within each host. Several regulatory proteins are known to be essential for the bacterium to complete these critical tasks, but interactions between regulators had not previously been investigated in detail, due to experimental uses of different strain backgrounds and growth conditions. To address that deficit in knowledge, the transcriptomic impacts of four critical regulatory proteins were examined in a uniform strain background. Pairs of mutants and their wild-type parent were grown simultaneously under a single, specific culture condition, permitting direct comparisons between the mutant strains. Transcriptomic analyses were strand-specific, and assayed both coding and noncoding RNAs. Intersection analyses identified regulatory overlaps between regulons, including transcripts involved in carbohydrate and polyamine metabolism. In addition, it was found that transcriptional units such as ospC and dbpBA, which w...Continue Reading

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Citations

May 7, 2019·Frontiers in Microbiology·John R CaskeyMonica E Embers
Sep 16, 2020·Infection and Immunity·Danielle E GrahamJon S Blevins
Mar 5, 2021·Current Protocols·J SeshuTaylor MacMackin Ingle
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Oct 21, 2020·Trends in Genetics : TIG·Evelyne Ng Kwan LimEric Massé
Dec 14, 2021·PloS One·Theerapat PhokaKanitha Patarakul

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Methods Mentioned

BETA
RNA-Seq
reverse
PCR
dissection

Software Mentioned

Salmon
Trimmomatic
Artemis
DESeq2
FASTA
Adobe Illustrator
GraphPad Prism
DESeq
ggplot2
BWA

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