Transcriptomic profiles of skeletal muscle tissue following an euglycemic-hyperinsulinemic clamp in insulin-resistant obese subjects.

Genes & Nutrition
Iwona RudkowskaMarie-Claude Vohl

Abstract

Insulin resistance in skeletal muscle is an early phenomenon in the pathogenesis of type 2 diabetes. Muscle is mainly responsible for insulin-stimulated glucose clearance from the bloodstream. Thus, regulation of gene expression in muscle tissue may be involved in the pathogenesis of insulin resistance. The objective was to investigate gene expression and metabolic pathways alterations in skeletal muscle tissue following an euglycemic-hyperinsulinemic clamp in obese insulin-resistant subjects. We carried out a transcriptome comparison of skeletal muscle tissue before and after a 3-h euglycemic-hyperinsulinemic clamp following 8-week supplementation with n-3 polyunsaturated fatty acid (PUFA) (1.8 g/day) with or without a supplement of fish gelatin (FG) (25 % of daily protein intake) in 16 obese insulin-resistant subjects. Results indicate that approximately 5 % (1932) of expressed transcripts were significantly changed after the clamp in both n-3 PUFA and n-3 PUFA + FG supplementation periods. Of these differentially expressed transcripts, 1394 genes associated with enzymes, transcription and translation regulators, transporters, G protein-coupled receptors, cytokines, and ligand-dependent nuclear receptors were modified. Metabo...Continue Reading

References

Aug 10, 1989·The New England Journal of Medicine·J ErikssonL Groop
Jan 1, 1996·The Journal of Clinical Investigation·A BrunettiI D Goldfine
Dec 1, 1995·Diabetologia·H Yki-Järvinen
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
May 23, 2001·JAMA : the Journal of the American Medical Association·UNKNOWN Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults
Nov 2, 2002·Nature Medicine·Joyce J Repa, David J Mangelsdorf
Nov 5, 2002·The Journal of Biological Chemistry·Guoqing CaoGarret J Etgen
Jan 29, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ute ZeitzReinhold G Erben
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Mary Elizabeth PattiLawrence J Mandarino
Nov 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Karine ClémentDominique Langin
Jul 18, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ian P J AlwaynMark Puder
Mar 13, 2008·American Journal of Physiology. Endocrinology and Metabolism·Dawn K ColettaChristopher P Jenkinson
Aug 13, 2008·Endocrine Reviews·Roger BouillonMarie Demay
Sep 21, 2010·Diabetes & Metabolism·C GarciaH Mayaudon

❮ Previous
Next ❯

Citations

May 2, 2014·Frontiers in Physiology·Patsie Polly, Timothy C Tan
Apr 21, 2016·Circulation. Cardiovascular Genetics·Jane F FergusonUNKNOWN American Heart Association Council on Functional Genomics and Translational Biology, Council on Epidemiology and Prevention,

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.