Transcriptomic Response of Chinese Yew (Taxus chinensis) to Cold Stress

Frontiers in Plant Science
Delong MengDiqiang Li

Abstract

Taxus chinensis is a rare and endangered shrub, highly sensitive to temperature changes and widely known for its potential in cancer treatment. How gene expression of T. chinensis responds to low temperature is still unknown. To investigate cold response of the genus Taxus, we obtained the transcriptome profiles of T. chinensis grown under normal and low temperature (cold stress, 0°C) conditions using Illumina Miseq sequencing. A transcriptome including 83,963 transcripts and 62,654 genes were assembled from 4.16 Gb of reads data. Comparative transcriptomic analysis identified 2,025 differently expressed (DE) isoforms at p < 0.05, of which 1,437 were up-regulated by cold stress and 588 were down-regulated. Annotation of DE isoforms indicated that transcription factors (TFs) in the MAPK signaling pathway and TF families of NAC, WRKY, bZIP, MYB, and ERF were transcriptionally activated. This might have been caused by the accumulation of secondary messengers, such as reactive oxygen species (ROS) and Ca(2+). While accumulation of ROS will have caused damages to cells, our results indicated that to adapt to low temperatures T. chinensis employed a series of mechanisms to minimize these damages. The mechanisms included: (i) cold-enh...Continue Reading

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Citations

May 8, 2019·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Guang-Xin EYan-Bin Zhu
Oct 18, 2018·International Journal of Molecular Sciences·Qiang ZhouZhipeng Liu
Jun 6, 2019·International Journal of Molecular Sciences·Chunlai WuWei Hu
Jan 21, 2021·International Journal of Molecular Sciences·Ziqing HeTing Wang

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Datasets Mentioned

BETA
SRR5167057
PRJNA360896

Methods Mentioned

BETA
Illumina sequencing
ELISA
RNA-seq
PCR
transgenic

Software Mentioned

BinGo
Trinity scripts
Trinity
Fastqc
Bioconductor R package
RSEM
TrinityStats
bowtie
cutadapt
Minitab

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