Transcriptomic response of durum wheat to cold stress at reproductive stage

Molecular Biology Reports
Marina L DíazAlicia Carrera

Abstract

Understanding the genetic basis of cold tolerance is a key step towards obtaining new and improved crop varieties. Current geographical distribution of durum wheat in Argentina exposes the plants to frost damage when spikes have already emerged. Biochemical pathways involved in cold tolerance are known to be early activated at above freezing temperatures. In this study we reported the transcriptome of CBW0101 spring durum wheat by merging data from untreated control and cold (5 °C) treated plant samples at reproductive stage. A total of 128,804 unigenes were predicted. Near 62% of the unigenes were annotated in at least one database. In total 876 unigenes were differentially expressed (DEGs), 562 were up-regulated and 314 down-regulated in treated samples. DEGs are involved in many critical processes including, photosynthetic activity, lipid and carbohydrate synthesis and accumulation of amino acids and seed proteins. Twenty-eight transcription factors (TFs) belonging to 14 families resulted differentially expressed from which eight families comprised of only TFs induced by cold. We also found 31 differentially expressed Long non-coding RNAs (lncRNAs), most of them up-regulated in treated plants. Two of these lncRNAs could oper...Continue Reading

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Methods Mentioned

BETA
RNA-seq
dissection
electrophoresis
PCR
environmental stress

Software Mentioned

GREENC
GeneChip
TrEMBL
DESeq2
FastQC
InfoStat
BLAST Onthology
BLAST
Web Gene Ontology Annotation Plot ( WEGO )
R package DEGseq

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