Transcriptomic Signatures of Postnatal and Adult Intrinsically Photosensitive Ganglion Cells

ENeuro
Daniel J BergDavid M Berson

Abstract

Intrinsically photosensitive retinal ganglion cells (ipRGCs) are rare mammalian photoreceptors essential for non-image-forming vision functions, such as circadian photoentrainment and the pupillary light reflex. They comprise multiple subtypes distinguishable by morphology, physiology, projections, and levels of expression of melanopsin (Opn4), their photopigment. The molecular programs that distinguish ipRGCs from other ganglion cells and ipRGC subtypes from one another remain elusive. Here, we present comprehensive gene expression profiles of early postnatal and adult mouse ipRGCs purified from two lines of reporter mice that mark different sets of ipRGC subtypes. We find dozens of novel genes highly enriched in ipRGCs. We reveal that Rasgrp1 and Tbx20 are selectively expressed in subsets of ipRGCs, though these molecularly defined groups imperfectly match established ipRGC subtypes. We demonstrate that the ipRGCs regulating circadian photoentrainment are diverse at the molecular level. Our findings reveal unexpected complexity in gene expression patterns across mammalian ipRGC subtypes.

Citations

Jul 28, 2020·Biology·Russell G FosterStuart N Peirson
Dec 17, 2020·Frontiers in Molecular Neuroscience·Julia SchaefferHomaira Nawabi
Apr 4, 2021·Experimental Eye Research·Michael J GilhooleyMark W Hankins
Apr 13, 2021·Frontiers in Neurology·Ludovic S Mure
Jun 8, 2021·Archivos De La Sociedad Española De Oftalmología·B Vidal-VillegasM Vidal-Sanz

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Datasets Mentioned

BETA
GSE118780

Methods Mentioned

BETA
transgenic
FACS
fluorescence-activated cell sorting
nucleotide exchange
RNA assay
RNA-seq
MDS
GTPase
scRNA-seq

Software Mentioned

GENSAT
quality
fastx
filter
DAVID
Covaris
ImageJ
fastq
SAM
EdgeR

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