Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.)

PeerJ
Weiping ShiJie Guo

Abstract

Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular mechanisms responsible for drought stress responses is vital to improvement of agricultural production. In this study, we exposed the drought resistant F1 hybrid, M79, and its parental lines E1 and H1 to drought stress. Subsequent physiological analysis demonstrated that M79 showed higher photosynthetic energy conversion efficiency and drought tolerance than its parents. A transcriptomic study using leaves collected six days after drought treatment, when the soil water content was about ∼20%, identified 3066, 1895, and 2148 differentially expressed genes (DEGs) in M79, E1 and H1 compared to the respective untreated controls, respectively. Further analysis revealed 17 Gene Ontology (GO) enrichments and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in M79, including photosystem II (PSII) oxygen-evolving complex, peroxidase (POD) activity, plant hormone signal transduction, and chlorophyll biosynthesis. Co-regulation analysis suggested that these DEGs in M79 contribute...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 18, 2004·Journal of Plant Physiology·Attipalli Ramachandra ReddyMunusamy Vivekanandan
May 4, 2006·Annual Review of Plant Biology·Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki
Dec 26, 2006·Plant Physiology·Wan-Hong CaoJin-Song Zhang
Jul 3, 2007·Journal of Plant Physiology·Eliane Cristina Gruszka VendruscoloLuiz Gonzaga Esteves Vieira
Dec 14, 2007·Nucleic Acids Research·Minoru KanehisaYoshihiro Yamanishi
Mar 11, 2008·Current Opinion in Plant Biology·Matthew Reynolds, Roberto Tuberosa
Apr 29, 2008·Trends in Plant Science·Mike PollardJohn B Ohlrogge
Sep 11, 2009·Current Opinion in Plant Biology·Tomás Werner, Thomas Schmülling
Feb 23, 2010·Biochemical and Biophysical Research Communications·Charu LataManoj Prasad
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Victoria BonnecarrèreJorge Monza
Apr 5, 2011·Journal of Experimental Botany·Pinghua Li, Thomas P Brutnell
May 18, 2011·The Plant Journal : for Cell and Molecular Biology·Camila CaldanaMatthew A Hannah
May 15, 2012·Nature Biotechnology·Jeffrey L BennetzenKatrien M Devos
Sep 19, 2012·Critical Reviews in Biotechnology·Charu LataManoj Prasad
Nov 8, 2012·Nature Methods·Rintaro SaitoTrey Ideker
Dec 10, 2013·Annual Review of Plant Biology·Honghong Hu, Lizhong Xiong
Dec 20, 2013·The Journal of Experimental Biology·Ellen H ColebrookPeter Hedden
Mar 25, 2014·Plant Physiology and Biochemistry : PPB·Peter D MabbittJulian J Eaton-Rye
May 8, 2014·Journal of Experimental Botany·Benjamin A BabstChung-Jui Tsai

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
electrophoresis
chip
PCR
environmental stress

Software Mentioned

TermFinder
gplots
Cuffdiff
Cytoscape
SPSS
Tophat2
R
Cufflinks
Bioconductor package

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.