Transcriptomic variation of locally-infected skin of Epinephelus coioides reveals the mucosal immune mechanism against Cryptocaryon irritans

Fish & Shellfish Immunology
Yazhou HuXiao-Chun Luo

Abstract

Fish skin is the largest immunologically active mucosal organ, providing first-line defense against external pathogens. However, the skin-associated immune mechanisms of fish are still unclear. Cryptocaryon irritans is an obligate ectoparasitic ciliated protozoan that infects almost all marine fish, and is believed to be an excellent pathogen model to study fish mucosal immunity. In this study, a de novo transcriptome assembly of Epinephelus coioides skin post C. irritans tail-infection was performed for the first time using the Illumina HiSeq™ 2500 system. Comparative analyses of infected skin (group Isk) and uninfected skin (group Nsk) from the same challenged fish and control skin (group C) from uninfected control fish were conducted. As a result, a total of 91,082 unigenes with an average length of 2880 base pairs were obtained and among them, 38,704 and 48,617 unigenes were annotated based on homology with matches in the non-redundant and zebrafish database, respectively. Pairwise comparison resulted in 10,115 differentially-expressed genes (DEGs) in the Isk/C group comparison (4,983 up-regulated and 5,132 down-regulated), 2,275 DEGs in the Isk/Nsk group comparison (1,319 up-regulated and 956 down-regulated) and 4,566 DEGs...Continue Reading

Citations

Apr 11, 2020·Science·Maria Rescigno
Aug 2, 2020·Parasite Immunology·Mateusz KonczalMagdalena Chadzinska
Jan 1, 2021·Frontiers in Immunology·Babak NajafpourDeborah M Power
Feb 21, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Quanquan CaoShaowu Yin
Mar 2, 2021·Veterinary Immunology and Immunopathology·Omkar ByadgiPaola Beraldo

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