Abstract
Fish skin is the largest immunologically active mucosal organ, providing first-line defense against external pathogens. However, the skin-associated immune mechanisms of fish are still unclear. Cryptocaryon irritans is an obligate ectoparasitic ciliated protozoan that infects almost all marine fish, and is believed to be an excellent pathogen model to study fish mucosal immunity. In this study, a de novo transcriptome assembly of Epinephelus coioides skin post C. irritans tail-infection was performed for the first time using the Illumina HiSeq™ 2500 system. Comparative analyses of infected skin (group Isk) and uninfected skin (group Nsk) from the same challenged fish and control skin (group C) from uninfected control fish were conducted. As a result, a total of 91,082 unigenes with an average length of 2880 base pairs were obtained and among them, 38,704 and 48,617 unigenes were annotated based on homology with matches in the non-redundant and zebrafish database, respectively. Pairwise comparison resulted in 10,115 differentially-expressed genes (DEGs) in the Isk/C group comparison (4,983 up-regulated and 5,132 down-regulated), 2,275 DEGs in the Isk/Nsk group comparison (1,319 up-regulated and 956 down-regulated) and 4,566 DEGs...Continue Reading
Citations
Jan 19, 2018·International Journal of Molecular Sciences·Arun SudhagarMansour El-Matbouli
Nov 22, 2017·Parasites & Vectors·Fei Yin, Dong Qian
Apr 11, 2020·Science·Maria Rescigno
Aug 2, 2020·Parasite Immunology·Mateusz KonczalMagdalena Chadzinska
Sep 5, 2020·Journal of Fish Biology·Jie BaiYuchen Niu
Dec 1, 2019·Parasites & Vectors·Arun SudhagarMansour El-Matbouli
Oct 21, 2020·Biology·Alejandra ValleJesús Lamas
Jan 1, 2021·Frontiers in Immunology·Babak NajafpourDeborah M Power
Dec 24, 2019·Fish & Shellfish Immunology·Liqin JiYing Liu
Oct 24, 2020·Parasitology Research·Shun ZhouXiaohui Ai
Dec 15, 2020·Journal of Fish Diseases·Jian-Xin ChengYing Liu
Feb 22, 2021·Microbial Pathogenesis·Shaowei ZhaiSonglin Guo
Feb 21, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Quanquan CaoShaowu Yin
Mar 2, 2021·Veterinary Immunology and Immunopathology·Omkar ByadgiPaola Beraldo
Jun 9, 2020·Fish & Shellfish Immunology·Jie BaiJingfeng Sun
Apr 30, 2021·Journal of Fish Diseases·Xiao XieFei Yin