Transcriptomics analysis defines global cellular response of Agrobacterium tumefaciens 5A to arsenite exposure regulated through the histidine kinases PhoR and AioS

Environmental Microbiology
Rachel A RawleTimothy R McDermott

Abstract

In environments where arsenic and microbes coexist, microbes are the principal drivers of arsenic speciation, which directly affects bioavailability, toxicity and bioaccumulation. Speciation reactions influence arsenic behaviour in environmental systems, directly affecting human and agricultural exposures. Arsenite oxidation decreases arsenic toxicity and mobility in the environment, and therefore understanding its regulation and overall influence on cellular metabolism is of significant interest. The arsenite oxidase (AioBA) is regulated by a three-component signal transduction system AioXSR, which is in turn regulated by the phosphate stress response, with PhoR acting as the master regulator. Using RNA-sequencing, we characterized the global effects of arsenite on gene expression in Agrobacterium tumefaciens 5A. To further elucidate regulatory controls, mutant strains for histidine kinases PhoR and AioS were employed, and illustrate that in addition to arsenic metabolism, a host of other functional responses are regulated in parallel. Impacted functions include arsenic and phosphate metabolism, carbohydrate metabolism, solute transport systems and iron metabolism, in addition to others. These findings contribute significantly...Continue Reading

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Citations

Nov 28, 2019·Environmental Microbiology Reports·Timothy R McDermottRonald S Oremland
Dec 29, 2020·Applied and Environmental Microbiology·Weiping ZhuangBrian Bothner
Mar 23, 2021·Frontiers in Microbiology·Rachel RawleTimothy R McDermott
Apr 27, 2021·Computational and Structural Biotechnology Journal·Miguel A MatillaTino Krell

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