Transcriptomics of cardiac biopsies reveals differences in patients with or without diagnostic parameters for heart failure with preserved ejection fraction

Scientific Reports
Sarbashis DasBengt Persson

Abstract

Heart failure affects 2-3% of adult Western population. Prevalence of heart failure with preserved left ventricular (LV) ejection fraction (HFpEF) increases. Studies suggest HFpEF patients to have altered myocardial structure and functional changes such as incomplete relaxation and increased cardiac stiffness. We hypothesised that patients undergoing elective coronary bypass surgery (CABG) with HFpEF characteristics would show distinctive gene expression compared to patients with normal LV physiology. Myocardial biopsies for mRNA expression analysis were obtained from sixteen patients with LV ejection fraction ≥45%. Five out of 16 patients (31%) had echocardiographic characteristics and increased NTproBNP levels indicative of HFpEF and this group was used as HFpEF proxy, while 11 patients had Normal LV physiology. Utilising principal component analysis, the gene expression data clustered into two groups, corresponding to HFpEF proxy and Normal physiology, and 743 differentially expressed genes were identified. The associated top biological functions were cardiac muscle contraction, oxidative phosphorylation, cellular remodelling and matrix organisation. Our results also indicate that upstream regulatory events, including inhibi...Continue Reading

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Citations

Sep 11, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Moritz Lindquist LiljeqvistJoy Roy
Aug 13, 2020·Current Heart Failure Reports·Jan D LanzerJulio Saez-Rodriguez
Apr 20, 2021·Computational and Structural Biotechnology Journal·Yongqing WuYuehua Cui
Jun 3, 2021·International Journal of Molecular Sciences·Emily S WiresBrandon K Harvey
Jun 21, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Ruchita ShandilyaPradyumna Kumar Mishra
Dec 22, 2021·The Journal of Biological Chemistry·Tram Anh TranElisabeth D Martinez

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Methods Mentioned

BETA
coronary
biopsies
RNA-seq
biopsy
PCA
exome sequencing
RNAseq

Software Mentioned

HiSeq Control
CASAVA
ARSyNseq
NOIseqbio
SIMCA
Star Aligner
NOISeq
FastQC
bcl2fastq
Ingenuity Pathway Analysis ( IPA

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