Transcriptomics profiling of Indian mustard (Brassica juncea) under arsenate stress identifies key candidate genes and regulatory pathways

Frontiers in Plant Science
Sudhakar SrivastavaPenna Suprasanna

Abstract

Arsenic (As) is a non-essential element, a groundwater pollutant, whose uptake by plants produces toxic effects. The use of As-contaminated groundwater for irrigation can affect the crop productivity. Realizing the importance of the Brassica juncea as a crop plant in terms of oil-yield, there is a need to unravel mechanistic details of response to As stress and identify key functional genes and pathways. In this research, we studied time-dependent (4-96 h) transcriptome changes in roots and shoots of B. juncea under arsenate [As(V)] stress using Agilent platform. Among the whole transcriptome profiled genes, a total of 1,285 genes showed significant change in expression pattern upon As(V) exposure. The differentially expressed genes were categorized to various signaling pathways including hormones (jasmonate, abscisic acid, auxin, and ethylene) and kinases. Significant effects were also noticed on genes related to sulfur, nitrogen, CHO, and lipid metabolisms along with photosynthesis. Biochemical assays were conducted using specific inhibitors of glutathione and jasmonate biosynthesis, and kinases. The inhibitor studies revealed interconnection among sulfur metabolism, jasmonate, and kinase signaling pathways. In addition, vari...Continue Reading

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Citations

Jul 6, 2017·Frontiers in Plant Science·Surabhi AwasthiRudra D Tripathi
Jan 25, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sandra Ndagire KamenyaDamaris Achieng Odeny
Dec 2, 2020·Trends in Plant Science·Shanti S SharmaKarl-Josef Dietz

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Datasets Mentioned

BETA
GSE66464

Methods Mentioned

BETA
in vitro transcription
chip
Feature Extraction
PCR
environmental stress

Software Mentioned

Feature Extraction Feature Extraction
Superviewer
Agilent Technologies Feature Extraction
STEM
PlantGSEA
CAP3
Feature Extraction
MCS
REST
Mapman

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