Transcriptomics tool for the human Schistosoma blood flukes using microarray gene expression profiling

Experimental Parasitology
Geoffrey N GobertDonald P McManus

Abstract

We report the design, fabrication, and validation of a microarray covering the majority of the Schistosoma japonicum and Schistosoma mansoni transcriptomes. The oligonucleotide microarray contains 12,166 S. mansoni and 7055 S. japonicum target sequences. A confidence threshold of <or=0.001 (P value) was used in all analyses. The cross hybridization potential of the microarray was representative of 10,362 probes detected in both channels, while 12,052 probes hybridised to S. japonicum and 11,254 probes hybridised to S. mansoni. Differential hybridisation consisted of 3422 probes to S. mansoni mRNA and 3103 probes to S. japonicum mRNA. Important biological functions, such as transport, metabolism, immune evasion and host-parasite immunological interplay, cell communication, and sexual maturation are represented on this microarray. This is the first microarray commercially manufactured for studying schistosomes, and the large size and verified quality of the resource demonstrate its power for characterising the schistosome transcriptome.

References

Jan 1, 1995·Pharmacology & Therapeutics·D CioliS Archer
Apr 19, 2002·The New England Journal of Medicine·Allen G P RossDonald P McManus
Aug 9, 2003·DNA and Cell Biology·David A Morrison, John T Ellis
Sep 16, 2003·Nature Genetics·Sergio Verjovski-AlmeidaEmmanuel Dias-Neto
Oct 2, 2003·Trends in Parasitology·John C BoothroydUpinder Singh
Nov 25, 2003·Analytical Chemistry·Thomas L FareYanqun Wang
Jun 24, 2004·Pharmacogenomics·Gary Hardiman
Aug 31, 2004·Nucleic Acids Research·Carole L YaukGeorge R Douglas
Oct 14, 2004·Molecular and Biochemical Parasitology·Jennifer M FitzpatrickKarl F Hoffmann
Sep 22, 2005·Parasitology·G N GobertD P McManus

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Citations

Jul 28, 2009·Annual Review of Genomics and Human Genetics·Ze-Guang HanZhu Chen
Apr 3, 2008·The Journal of Clinical Investigation·Peter J HotezJulie Jacobson
Mar 27, 2009·BMC Genomics·Geoffrey N GobertDonald P McManus
Jun 21, 2007·BMC Genomics·Michael WaisbergNajib M El-Sayed
Jan 12, 2012·PLoS Neglected Tropical Diseases·John N Milligan, Emmitt R Jolly
Jul 24, 2008·European Heart Journal·Archana Singh-ManouxMartin J Shipley
Oct 1, 2007·Expert Opinion on Drug Discovery·Matthew BerrimanJames P McCarter
Oct 2, 2012·Molecular and Biochemical Parasitology·Jarrett Hines-KayCharles Cunningham
Apr 27, 2010·Experimental Parasitology·Cibele Aparecida TararamLuciana Cezar de Cerqueira Leite
Dec 23, 2008·Molecular and Biochemical Parasitology·Anthony D AragonCharles Cunningham
Sep 30, 2008·Experimental Parasitology·M WaisbergG R Franco
Jun 26, 2008·Acta Tropica·Sergio C OliveiraLuciana C C Leite
Jul 13, 2007·Experimental Parasitology·Guilherme Oliveira
Jun 20, 2007·Experimental Parasitology·David L WilliamsTimothy P Yoshino
Jun 20, 2007·Experimental Parasitology·Jaap J van HellemondAloysius G M Tielens
Jul 8, 2010·Trends in Parasitology·Geoffrey N Gobert
Mar 6, 2007·Trends in Parasitology·Cornelis H HokkeKarl F Hoffmann
Sep 9, 2008·Molecular and Biochemical Parasitology·Anthony D AragonCharles Cunningham
Jan 1, 2015·Microarrays·Amir SyahirHisakazu Mihara
Dec 13, 2016·Expert Review of Proteomics·Javier SotilloAlex Loukas
Aug 7, 2021·Vaccines·Ndibonani Kebonang QokoyiAbidemi Paul Kappo

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