Transcripts at the mating type locus of Cochliobolus heterostrophus

Molecular & General Genetics : MGG
G Leubner-MetzgerB G Turgeon

Abstract

The single mating type locus (MAT) of the heterothallic ascomycete Cochliobolus heterostrophus is composed of a pair of unlike sequences called idiomorphs, each of which encodes one MAT-specific gene (MAT-1 and MAT-2). MAT transcripts were observed in blots of poly(A)+ RNA isolated from cultures grown in minimal medium, but were not detectable after growth of the fungus in complete medium, suggesting that transcription of MAT is tightly regulated. The idiomorphs (MAT-1 = 1297-bp, MAT-2 = 1171-bp) encode transcripts of 2.2 kb (MAT-1) and 2.1 kb (MAT-2), which start 5' and end 3' of the idiomorph within sequences common to both mating types. Analyses of MAT-1 and MAT-2 cDNAs revealed obligatory splicing of one intron (55-bp in MAT-1, 52-bp in MAT-2) within each MAT-specific ORF and optional splicing of two introns (63 and 79-bp) in the long (approximately 0.55 kb) 5' untranslated leader sequences; the 3' untranslated region is 0.46 kb long. Transcription start sites were found 5' of, and within, the 79-bp intron. Optional splicing of the upstream introns and at least two transcription start sites result in three types of transcript: Type I with both 5' introns spliced, Type II with only the 63-bp intron spliced, and Type III with...Continue Reading

Citations

Mar 12, 2004·Annual Review of Phytopathology·B G Turgeon
Jul 3, 2007·Revista iberoamericana de micología·Laura Conde-Ferráez
Dec 16, 2000·Fungal Genetics and Biology : FG & B·B G Turgeon, O C Yoder
Jul 22, 1999·Fungal Genetics and Biology : FG & B·M PuyeskyB A Horwitz

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