Transcripts Encoding the Androgen Receptor and IGF-Related Molecules Are Differently Expressed in Human Granulosa Cells From Primordial and Primary Follicles

Frontiers in Cell and Developmental Biology
Line L SteffensenKarin Lykke-Hartmann

Abstract

Bidirectional cross talk between granulosa cells and oocytes is known to be important in all stages of mammalian follicular development. Insulin-like growth factor (IGF) signaling is a prominent candidate to be involved in the activation of primordial follicles, and may be be connected to androgen-signaling. In this study, we interrogated transcriptome dynamics in granulosa cells isolated from human primordial and primary follicles to reveal information of growth factors and androgens involved in the physiology of ovarian follicular activation. Toward this, a transcriptome comparison study on primordial follicles (n = 539 follicles) and primary follicles (n = 261 follicles) donated by three women having ovarian tissue cryopreserved before chemotherapy was performed. The granulosa cell contribution in whole follicle isolates was extracted in silico. Modeling of complex biological systems was performed using IPA® software. We found the granulosa cell compartment of the human primordial and primary follicles to be extensively enriched in genes encoding IGF-related factors, and the Androgen Receptor (AR) enriched in granulosa cells of primordial follicles. Our study hints the possibility that primordial follicles may indeed be andr...Continue Reading

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Citations

May 12, 2021·Molecular Human Reproduction·E R FrostJ M Sutherland

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Methods Mentioned

BETA
Nucleotide Exchange
GTPase
Laser Capture Microdissection
RNA-Seq

Software Mentioned

SSCEGs
IPA®
IPA
Ingenuity Pathway Analysis ( IPA® )
Cufflinks
Zen
BWA
ImageJ
R
Tophat

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