Transcripts of neurokinin B and neurokinin 3 receptor in superovulated rat ovaries and increased number of corpora lutea as a non-specific effect of intraperitoneal agonist application

Regulatory Peptides
Sabine LöfflerKatharina Spanel-Borowski

Abstract

Neurokinin B (NKB), a member of the tachykinin family, and its neurokinin 3 receptor (NK3-R) are preferentially found in the central nervous system. Others have recently reported on mRNA from this ligand-receptor system in the uterus and on NK3-R expression increasing with age. NKB and NK3-R mRNAs have also been noted in cumulus cells and oocytes from superovulated rats. Intact ovaries before and after puberty have not been studied. In this study, we stimulated 29-day-old rats by s.c. injections with gonadotropins for estrous cycle synchronization in order to elucidate the NKB-NK3-R system's expression and function in the ovary. Simultaneously, NaCl, the NK3-R agonist (Pro(7))-NKB, the antagonist SB 218795, or thiorphan, a neutral endopeptidase inhibitor of tachykinin degradation, were injected intraperitoneally (i.p.) for 3 1/2 consecutive days. First, we demonstrated NKB and NK3-R transcripts in one rat ovary by RT-PCR. No significant mRNA differences were noted between immature ovaries and superovulated ovaries in any of the i.p. applications. Second, the possible role of NK3-R on the ovulatory process was verified by counting corpora lutea (CL) and CL cysts in serial sections of the other ovary derived from the four differe...Continue Reading

References

Feb 1, 1995·Obstetrics and Gynecology·R W StonesG Burnstock
Mar 11, 2000·Human Reproduction Update·O Bukulmez, A Arici
Nov 9, 2000·Clinical and Experimental Pharmacology & Physiology·E N PatakM E Story
Feb 13, 2001·Journal of Internal Medicine·T HökfeltJ Wahren
Mar 7, 2001·Regulatory Peptides·N M PageP J Lowry
Aug 15, 2001·European Journal of Pharmacology·F M PintoM L Candenas
Nov 8, 2001·European Journal of Pharmacology·R Patacchini, C A Maggi
May 31, 2002·Pharmacological Reviews·Cinzia SeveriniVittorio Erspamer
Oct 19, 2002·Journal of Reproductive Immunology·Mats Brännström, Anders Enskog
Oct 23, 2002·Biology of Reproduction·Linda H CraneGary P Hamlin
Jan 28, 2003·American Journal of Obstetrics and Gynecology·Mark WareingPhilip N Baker
May 30, 2003·Biology of Reproduction·C Oscar PintadoM Luz Candenas
Jun 6, 2003·British Journal of Pharmacology·Eva PatakFrancisco M Pinto
Jul 6, 2004·Molecular Reproduction and Development·Sabine LöfflerKatharina Spanel-Borowski

❮ Previous
Next ❯

Citations

Aug 31, 2014·General and Comparative Endocrinology·Guangfu HuAnderson O L Wong
Sep 17, 2005·Peptides·Luciano Debeljuk
Dec 18, 2010·Molecular and Cellular Endocrinology·Katharina Spanel-Borowski
Nov 10, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Agnes ZvaraPéter Ferdinandy
Jan 8, 2020·Journal of Clinical Medicine·Víctor BlascoManuel Fernández-Sánchez
Aug 14, 2019·The Journal of Reproduction and Development·Larasati Puji RahayuTomomi Tanaka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.