PMID: 15227723Jul 1, 2004Paper

Transdifferentiation of cultured bovine lens epithelial cells into myofibroblast-like cells by serum modulation

Yonsei Medical Journal
Jong-Tak KimChoun-Ki Joo

Abstract

An after-cataract is caused by the proliferation of residual cells over the equator of the lens. These cells subsequently migrate to the posterior lens capsule, where they undergo aberrant differentiation into fiber-like cells or transdifferentiation into fibroblast-like cells. To study the precise molecular mechanisms of transdifferentiation, an attempt was made to establish an in vitro system, in which the lens epithelial cells (LECs) of the pre-equatorial zone could be transdifferentiated into fibroblast-like cells. The required conditions for culturing the LECs were identified as consisting of four phases; intact bovine explants, explant-cultured, serum-modulated and additionally modulated LECs. The LECs of each phase were compared by examining changes in the expression of the epithelial-mesenchymal transition (EMT)-related genes and changes in cellular morphology and adhesion. The explants that were cultured in a medium containing 10% fetal bovine serum (FBS) for 2 weeks, showed changes in the expression of the EMT-related genes, although the other explant-cultured cells maintained an epithelial morphology. To introduce a transition into mesenchymal cells, the explant cultures were subcultured in a medium containing 20% FB...Continue Reading

Citations

Aug 2, 2008·Experimental Eye Research·Janice Walker, A Sue Menko
Aug 28, 2007·Journal of Cataract and Refractive Surgery·Miran Rhee, Thomas A Oetting
Apr 3, 2007·Journal of Cataract and Refractive Surgery·Matthias PollhammerFriedrich E Kruse
Dec 1, 2006·Seminars in Cell & Developmental Biology·Elizabeth D Wederell, Robb U de Iongh
Aug 31, 2017·Journal of Cell Science·Stephanie R GillespieAudrey M Bernstein
Jun 29, 2012·Tissue Engineering. Part a·Derek H RosenzweigThomas M Quinn
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Robin AugustineAla-Eddin Al Moustafa

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