PMID: 9636180Jun 24, 1998Paper

Transdominant genetic analysis of a growth control pathway

Proceedings of the National Academy of Sciences of the United States of America
G CaponigroA Kamb

Abstract

Genetic selections that use proteinaceous transdominant inhibitors encoded by DNA libraries to cause mutant phenocopies may facilitate genetic analysis in traditionally nongenetic organisms. We performed a selection for random short peptides and larger protein fragments (collectively termed "perturbagens") that inhibit the yeast pheromone response pathway. Peptide and protein fragment perturbagens that permit cell division in the presence of pheromone were recovered. Two perturbagens were derived from proteins required for pheromone response, and an additional two were derived from proteins that may negatively influence the pheromone response pathway. Furthermore, three known components of the pathway were identified as probable perturbagen targets based on physical interaction assays. Thus, by selection for transdominant inhibitors of pheromone response, multiple pathway components were identified either directly as gene fragments or indirectly as the likely targets of specific perturbagens. These results, combined with the results of previous work [Holzmayer, T. A., Pestov, D. G. & Roninson, I. B. (1992) Nucl. Acids. Res. 20, 711-717; Whiteway, M., Dignard, D. & Thomas, D. Y. (1992) Proc. Natl. Acad. Sci. USA 89, 9410-9414; a...Continue Reading

References

Mar 1, 1992·Molecular & General Genetics : MGG·N Firth, R Skurray
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M WhitewayD Y Thomas
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S W RamerR W Davis
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E A ElionG R Fink
Jan 1, 1990·Molecular and Cellular Biology·M WhitewayD Y Thomas
Aug 1, 1985·Molecular and Cellular Biology·D T Chaleff, K Tatchell
Apr 1, 1995·Current Opinion in Cell Biology·D E Levin, B Errede
Feb 23, 1995·Nature·R HeimR Y Tsien
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S MarcusM Wigler
Jan 1, 1993·Annual Review of Genetics·J Kurjan
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·A V GudkovI B Roninson
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·L LiR J Legerski
Oct 1, 1993·Molecular & General Genetics : MGG·C M McEnteeA P Hudson
Jan 1, 1993·Molecular and Cellular Biology·K L ClarkM Whiteway
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R PerlmanA Levitzki
Nov 22, 1996·Science·P DemantM Svobodova

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Citations

Oct 13, 2009·Current Genetics·Edward DraperDavid E Stone
Aug 2, 2003·Journal of Biotechnology·Giordano CaponigroAlexander Kamb
Jan 11, 2000·Research in Microbiology·D Ladant, G Karimova
Apr 21, 1999·Current Opinion in Biotechnology·E V ShustaK D Wittrup
Sep 22, 2001·The Journal of Steroid Biochemistry and Molecular Biology·F Hoppe-SeylerK Butz
Jan 28, 2003·Drug Discovery Today·Leodevico L IlagStefan W Henning
Feb 13, 2001·Current Opinion in Chemical Biology·A Kamb, G Caponigro
Feb 13, 2001·Current Opinion in Chemical Biology·S J BoultonM Vidal
Feb 19, 2000·Current Opinion in Chemical Biology·P Colas
Mar 20, 2001·Nature Reviews. Genetics·B R Stockwell
Dec 22, 1999·Nucleic Acids Research·M J FeldhausA Kamb
Aug 26, 2003·Antimicrobial Agents and Chemotherapy·R Edward BensonPaul T Hamilton
Feb 8, 2008·Journal of Biology·Pierre Colas
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·E L PerkinsM A Resnick
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·J H BlumJ J Mekalanos
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·C R GeyerR Brent
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·C P ScottS J Benkovic
Jun 15, 2005·Expert Opinion on Biological Therapy·Corina BorghoutsBernd Groner
Sep 12, 2002·Expert Opinion on Therapeutic Targets·Renuka C PillutlaNeil I Goldstein
Nov 10, 2000·Developmental Biology·M G Kolonin, R L Finley
Aug 22, 2001·Chemistry & Biology·C P ScottS J Benkovic
Sep 3, 2003·Virology·Mark A PoritzDavid H-F Teng
Sep 6, 2005·Journal of Molecular Biology·Robbie WoodmanPaul Ko Ferrigno
Dec 2, 2000·Nature Biotechnology·M Li
Sep 1, 2000·FEBS Letters·A ZucconiG Cesareni
Apr 15, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elisa Beghetto, Nicola Gargano
Sep 3, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Corina BorghoutsBernd Groner
Sep 13, 2005·Biotechnology and Bioengineering·Todd A NaumannStephen J Benkovic
Jan 4, 2001·Nature Genetics·X XuG P Nolan
Jul 13, 2002·Cytometry·Burt RichardsDavid H-F Teng
Mar 4, 2008·Molecular Cancer Research : MCR·Corina BorghoutsBernd Groner
Oct 21, 1999·Methods : a Companion to Methods in Enzymology·D R Appling
Aug 26, 1999·Trends in Biotechnology·M Vidal, H Endoh

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