PMID: 9447241Feb 3, 1998Paper

Transduction of antibiotic resistance markers among Actinobacillus actinomycetemcomitans strains by temperate bacteriophages Aa phi 23

Cellular and Molecular Life Sciences : CMLS
K WilliJürg Meyer

Abstract

Actinobacillus actinomycetemcomitans (Aa) strain ST1 carries the tetracycline (Tc) resistance transposon Tn916 and the Aa phi ST1 prophage, which is closely related to temperate bacteriophage Aa phi 23. High titre phage preparations were obtained from this strain by mitomycin C induction and were used to transduce the TcR determinant to the TcS recipient strains ZIB1001 and ZIB1015 (MIC 2 micrograms Tc/ml). TcR transductants (MIC > or = 32 micrograms Tc/ml) were detected at frequencies of 3 x 10(-6) to 5 x 10(-8) per pfu. All TcR transductants examined contained the entire Tn916 inserted at several different locations within the Aa genome. They appear to have resulted from generalized transduction. In addition both bacteriophages, Aa phi 23 and Aa phi ST1, were capable of transducing the chloramphenicol (Cm) resistance marker of plasmid pKT210 (transduction frequencies of 2 x 10(-5) to 3 x 10(-7) per pfu) to the recipient strain ZIB1001 (MIC 8 micrograms Cm/ml). Eleven CmR ZIB1001 transductants (MIC > or = 100 micrograms Cm/ml) studied carried a plasmid indistinguishable from pKT210 by restriction analyses. In view of the high prevalence of this phage family, and the increasing use of tetracycline in periodontitis therapy, thes...Continue Reading

Citations

Oct 30, 2013·Antimicrobial Agents and Chemotherapy·Pablo QuirósMaite Muniesa
Jun 23, 2004·Emerging Infectious Diseases·Maite MuniesaFerran Navarro
Apr 20, 2010·Biostatistics·Paola BortotBruno Scarpa
Dec 8, 2010·Expert Review of Anti-infective Therapy·Adam P Roberts, Peter Mullany
Aug 26, 2006·Periodontology 2000·Adam P Roberts, Peter Mullany
Jan 20, 2015·Plasmid·Maryury Brown-JaqueMaite Muniesa
Feb 26, 2015·Frontiers in Cellular and Infection Microbiology·Roy H Stevens
Sep 25, 2014·Frontiers in Cellular and Infection Microbiology·Adam P Roberts, Jens Kreth
Dec 4, 2014·International Journal of Antimicrobial Agents·Clarisse DupinAnne Jolivet-Gougeon
Mar 20, 2015·FEMS Microbiology Letters·Rachid MenouniMireille Ansaldi
Jun 7, 2013·Journal of Oral Microbiology·Ingar OlsenBing-Yan Wang
Jan 22, 2017·Journal of Biotechnology·Szymon P SzafrańskiMeike Stiesch
Sep 25, 2018·Canadian Journal of Microbiology·Nicole A Lerminiaux, Andrew D S Cameron
Jan 13, 2019·Dentistry Journal·Liviu SteierJosé Antonio Poli de Figueiredo
Aug 27, 2019·Journal of Oral Microbiology·Alexandre ArredondoRubén León
Apr 11, 2020·Frontiers in Microbiology·Rosalind A GilbertEleanor Jameson
Mar 11, 2021·Periodontology 2000·Szymon P SzafrańskiMeike Stiesch

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