Mar 1, 1976

Transduction of chromosomal genes between enteric bacteria by bacteriophage P1

Journal of Bacteriology
B M Tyler, R B Goldberg

Abstract

We have used P1 transduction to create intergeneric hybrid strains of enteric bacteria by moving the genA and hut genes between Klebsiella aerogenes, Escherichia coli and Salmonella typhimurium. The use of E. coli as the recipient in such transductions permits the construction of episomes and specialized transducing phage containing non-E. coli material. The effect of host restriction modification and deoxyribonucleic acid homology on the frequency of intergeneric transduction of these loci has been examined.

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Mentioned in this Paper

RNA Conformation
Alkalescens-Dispar Group
Histidine
RNA Viruses
Glutamate-Ammonia Ligase
Transduction, Genetic
Chromosomes, Bacterial
Cistron
Coliphages
DNA, Bacterial

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