Abstract
The generalized transducing bacteriophage of Salmonella typhimurium, P22, can transduce plasmids in addition to chromosomal markers. Previous studies have concentrated on transduction of pBR322 by P22 and P22HT, the high transducing mutant of P22. This study investigates the mechanism of P22HT transduction of low-copy number plasmids, namely pSC101 derivatives. We show that P22HT transduces pSC101 derivatives that share homology with the chromosome by two distinct mechanisms. In the first mechanism, the plasmid integrates into the chromosome of the donor by homologous recombination. This chromosomal fragment is then packaged in the transducing particle. The second mechanism is a size-dependent mechanism involving a putative plasmid multimer. We propose that this multimer is formed by interplasmidic recombination. In contrast, P22HT can efficiently transduce pBR322 by a third mechanism, which is independent of plasmid homology with the chromosome. It has been proposed that the phage packages a linear concatemer created during rolling circle replication of pBR322, similar in fashion to phage genome packaging. This study investigates the role of RecA, RecD, and RecF recombination proteins in plasmid/plasmid and plasmid/chromosome ...Continue Reading
Citations
Mar 15, 2005·BMC Microbiology·Jian QiaoLeonard Mindich
Mar 11, 2000·Molecular Microbiology·A Janakiraman, J M Slauch
Feb 7, 2006·Plasmid·Victor RavinTapani Alatossava
May 25, 2013·Future Microbiology·Maite MuniesaJuan Jofre
Oct 29, 2000·Journal of Bacteriology·C Vander Byl, A M Kropinski
Jul 29, 2020·The Journal of Antimicrobial Chemotherapy·Lorena Rodríguez-RubioBruno Gonzalez-Zorn
Jul 28, 2005·Molecular Microbiology·Craig D EllermeierJames M Slauch
Dec 16, 2004·FEMS Microbiology Letters·Sunhee LeeWilliam R Jacobs
May 31, 2014·Environmental Science & Technology·William Calero-CáceresMaite Muniesa
Oct 21, 2005·Journal of Bacteriology·Massimo MerighiJohn S Gunn
Sep 1, 2014·Genetics·Emiko SanoJohn R Roth
Sep 1, 2019·Applied and Environmental Microbiology·Adelumola OladeindeTravis Glenn
Oct 13, 1999·FEMS Immunology and Medical Microbiology·M L Kim, J M Slauch
Oct 26, 1999·Infection and Immunity·T C ZahrtS Maloy
Jan 3, 2001·Journal of Bacteriology·T D Ho, J M Slauch
Nov 17, 1999·Molecular Microbiology·H P ArnoldW Zillig
Jul 27, 2000·Journal of Bacteriology·T L StanleyJ M Slauch
Dec 4, 2019·Frontiers in Microbiology·Andrea I Moreno-SwittMagaly Toro
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·R A EdwardsS R Maloy
Feb 26, 2010·BMC Evolutionary Biology·Marco FondiRenato Fani
Nov 8, 2008·Blood·Thomas H YunJames H Morrissey
Oct 3, 2009·Microbiology·Dongxia LinJames M Slauch
Aug 23, 2011·PloS One·Yawei SunZonghui Yuan