Transduction of merodiploidy: induced duplication of recipient genes.

Journal of Bacteriology
C W HillP Berg

Abstract

Escherichia coli PB160, which carries a tandem duplication with the gene order metB(+)argH(-)su(159) (+)thi(+): metB(+)argH(+)su(159) (-)thi(+), was used to study the mechanism of P1 transduction of genes in the duplicated region. Transduction of the su(159) (+) allele contained within the duplicated segment yields two kinds of su(159) (+) recombinants: 91% are haploid su(159) (+) and 9% are su(159) (+)/su(159) (-) merodiploids. The duplication in these merodiploid transductants includes the metB locus; however, both copies of the metB locus usually are derived from the recipient. Thus, the requirements for transduction of the "condition of merodiploidy" appear to be the cotransduction of the repeat point (the region where the duplication begins to repeat itself) and, of course, the selected marker (in this case su(159) (+)). A mechanism whereby two recipient chromosomes interact with the transduced "repeat point" region to regenerate the tandem duplication is implicated. It appears that a duplication much larger than the quantity of genetic material carried by a P1 phage can be produced in a transductant.

Citations

Dec 31, 1973·Molecular & General Genetics : MGG·C W Hill, G Combriato
Jan 1, 1975·Molecular & General Genetics : MGG·T Mojica
Nov 17, 2014·Nucleic Acids Research·Yuta OtsukaHirotada Mori
Dec 1, 1972·Bacteriological Reviews·K B Low
Sep 1, 1976·Mutation Research·G E Murray, P F Smith-Keary
Nov 25, 1978·Journal of Molecular Biology·R P Anderson, J R Roth
May 28, 1971·Journal of Molecular Biology·T Boon, N D Zinder
Oct 14, 1970·Journal of Molecular Biology·J A SpudichC Yanofsky
Feb 7, 2001·Genetics·E M Camacho, J Casadesús

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