Transduction of peptide analogs of the small heat shock-related protein HSP20 inhibits intimal hyperplasia

Journal of Vascular Surgery
Deron J TessierColleen M Brophy

Abstract

Human saphenous vein (HSV) is the autologous conduit of choice for peripheral vascular reconstructions. However, vasospasm can lead to early graft failure. The leading cause of delayed graft failure is intimal hyperplasia. To develop a proteomic approach to prevent vein-graft spasm and intimal hyperplasia. Biomimetic peptide analogs of the small heat shock-related protein HSP20, containing a protein transduction domain (PTD), a phosphorylated serine, and a sequence of HSP20 surrounding the phosphorylation site (PTD-pHSP20), or a scrambled sequence of the same amino acids surrounding the phosphorylation site (PTD-scHSP20) were synthesized. The peptides were used in muscle bath and organ culture experiments with human saphenous vein (HSV) segments. Cultured smooth muscle cell lines were used to determine the effect of the peptides on proliferation and migration. In HSV rings precontracted with norepinephrine, PTD-pHSP20 but not PTD-scHSP20 led to relaxation. There was no significant difference in smooth muscle cell proliferation in cells treated with PTD-pHSP20 compared with PTD-scHSP20. Treatment with PTD-pHSP20 significantly inhibited cellular migration compared with PTD-scHSP20. Control, untreated, and PTD-scHSP20-treated saph...Continue Reading

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Citations

Jul 2, 2009·Cell Stress & Chaperones·Catherine M DreizaColleen M Brophy
Dec 12, 2012·American Journal of Respiratory Cell and Molecular Biology·Hiromichi AsoYoshinori Hasegawa
Nov 21, 2015·Vascular Pharmacology·Cressida A LyonSarah J George
Nov 7, 2006·Protein Expression and Purification·Charles R FlynnColleen M Brophy
Jun 28, 2005·Journal of the American College of Surgeons·Elisabeth C McLemoreColleen M Brophy
Jan 11, 2013·Autoimmune Diseases·Earl G Noble, Garry X Shen
Feb 26, 2016·American Journal of Respiratory Cell and Molecular Biology·Alex BanathyPadmini Komalavilas
Apr 2, 2017·Tropical Animal Health and Production·Rakesh KumarNishant Verma
Apr 21, 2017·The FEBS Journal·Boqian ZhuChengchun Tang
Oct 30, 2012·Vascular·Michael J OsgoodColleen Brophy
Oct 6, 2005·Journal of Muscle Research and Cell Motility·Olesya V BukachNikolai B Gusev
May 21, 2005·Pharmaceutical Research·Luciana B LopesM Vitoria L B Bentley
Oct 21, 2011·Physiological Reviews·Evgeny V MymrikovNikolai B Gusev
Jun 13, 2015·Journal of Applied Physiology·Tiegang LiuUsamah S Kayyali
Nov 13, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Padmini KomalavilasColleen M Brophy
Sep 19, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Kleiton Augusto Santos SilvaCraig A Emter
May 28, 2011·Cellular Signalling·H V EdwardsG S Baillie

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