Transection and Crush Models of Nerve Injury to Measure Repair and Remyelination in Peripheral Nerve

Methods in Molecular Biology
Xin-Peng Dun, David B Parkinson

Abstract

Injury to the peripheral nervous system begins a well-characterized process within both neurons and Schwann cells to allow axonal regrowth, remyelination, and functional repair. Models of peripheral nerve injury have been widely used to study the behavior of Schwann cells, neurons, and other cell types such as macrophages as the events of Wallerian degeneration and regeneration take place. The most commonly used approaches in rodent models to model nerve injury in human patients are sciatic nerve transection and nerve crush, and both have well established time courses of demyelination, immune cell influx, axonal regrowth, and remyelination. We describe the techniques of sciatic nerve surgery for transection and crush injury, together with methods for the analysis of events within peripheral nerve repair in these two models.

Citations

Dec 19, 2019·Annals of Neurology·Vincenzo ProviteraMaria Nolano
Jul 14, 2020·Journal of Cellular Physiology·Yunzhong ZhangXin Tang
Nov 14, 2019·Neural Regeneration Research·Bing ChenXin-Peng Dun
Jan 11, 2020·Frontiers in Molecular Neuroscience·Bing ChenXin-Peng Dun

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