Transepithelial paracellular leakiness induced by chronic phorbol ester exposure correlates with polyp-like foci and redistribution of protein kinase C-alpha

Carcinogenesis
J M MullinA P Soler

Abstract

Although exposure of LLC-PK1 epithelial cell sheets to phorbol esters (TPA) causes a near immediate and total decrease of transepithelial electrical resistance (TER), continuation of exposure for 3 to 4 days results in a tachyphylactic response as TER begins to return to control levels. Recovery of TER is maximal by 5 to 6 days, but reaches only 70 to 80% of control level. A reciprocal change in the transepithelial flux of D-mannitol indicates that the TER decrease is indicative of an increase in tight junction permeability. Exposure of cell sheets to TPA for several days also results in the appearance of multilayered polyp-like foci (PLFs) across the otherwise one cell layer thick cell sheets. The pattern of penetration of the electron dense dye, ruthenium red, from the apical surface, across the tight junction and into the lateral intercellular space indicates that the tight junctions of the cell sheet become uniformly leaky after acute exposure to TPA. However, when exposure is continued for several days, only the junctions of cells in the PLFs manifest leakiness. The decrease in TER following acute TPA exposure correlates with the translocation of protein kinase C-alpha (PKC alpha) into a membrane-associated compartment. Wi...Continue Reading

Citations

Nov 15, 2006·Digestive Diseases and Sciences·J M MullinJ J Thornton
Apr 12, 2005·Advanced Drug Delivery Reviews·Gemma J FeldmanMichael P Ryan
Jun 16, 2000·Advanced Drug Delivery Reviews·H ClarkeJ M Mullin
Jul 17, 1999·Pharmaceutical Science & Technology Today·A L Daugherty, R J Mrsny
May 23, 2007·Nutrition and Cancer·Chris I R GillIan Rowland
Sep 24, 2005·Molecular Biology of the Cell·James M MullinChristian Sell
Dec 23, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M LahnD Ma
Jul 31, 2010·Journal of Oncology·Amar B SinghPunita Dhawan
Apr 14, 2015·Seminars in Cancer Biology·W G JiangD Santini
Feb 23, 2000·The Journal of Cell Biology·D Li, R J Mrsny
May 10, 2005·International Journal of Cancer. Journal International Du Cancer·Chris I R GillIan Rowland
Dec 4, 2003·International Journal of Cancer. Journal International Du Cancer·Thorsten HoevelManfred Kubbies
Aug 8, 2008·Alimentary Pharmacology & Therapeutics·J M MullinJ J Thornton
Sep 29, 2011·Alcoholism, Clinical and Experimental Research·Samantha M SimetJoseph H Sisson
Oct 24, 2007·Biochimica Et Biophysica Acta·Lorenza González-MariscalDavid Chamorro
Jan 5, 2006·Canadian Journal of Physiology and Pharmacology·Toshiyuki YamagataYves Berthiaume
Aug 4, 1998·The American Journal of Physiology·J M MullinD Rosson

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