Transepithelial Transport Characteristics of the Cholesterol- Lowing Soybean Peptide, WGAPSL, in Caco-2 Cell Monolayers

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Huijuan ZhangJing Wang

Abstract

Recent studies have shown that soybean protein and its peptides have cholesterol-lowering activities. However, it is not clear whether these peptides could overcome physiological barriers, such as phase II metabolism in gastrointestinal tract and poor permeability, to reach the blood stream in its intact form. Therefore, the transepithelial transport characteristics of soybean peptide Trp-Gly-Ala-Pro-Ser-Leu (WGAPSL) with cholesterol- lowering activity were investigated in Caco-2 cells. In this study; the transepithelial absorption of WGAPSL was studied using human intestinal Caco-2 cell monolayers. The results showed that WGAPSL had good stability (83.9% ±1.98%) after simulated gastric and intestinal digestion. During the apical (AP) side to basolateral (BL) side transport, WGAPSL was absorbed intact through Caco-2 cell monolayers with apparent permeability coefficient (Papp) values of 4.4 × 10-8 to 1.2 × 10-8 cm/s. Cytochalasin D loosened the tight junctions of Caco-2 cell monolayers and significantly (p < 0.05) improved the transport process. Sodium azide, wortmannin, and Gly-Pro had minimal effects on transport, demonstrating that the major transport route of WGAPVL was paracellular via tight junctions. Finally, LC-MS analy...Continue Reading

References

Sep 1, 1990·Pharmaceutical Research·A R HilgersP S Burton
Mar 15, 1993·Annals of the New York Academy of Sciences·C R SirtoriM R Lovati
Mar 22, 2001·Advanced Drug Delivery Reviews·P ArturssonK Luthman
Jul 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·G A LEVEILLE, H FISHER
Jun 8, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Misako Aito-InoueYoshinori Mine
Dec 11, 2007·Journal of Proteome Research·Jesse RodriguezPavel A Pevzner
Apr 23, 2008·Current Protocols in Protein Science·B M Dunn
Apr 30, 2008·Journal of Agricultural and Food Chemistry·Lin LeiShimin Li
Jan 14, 2010·The FEBS Journal·Stefanie GeisslerMatthias Brandsch
Apr 17, 2010·Molecular Nutrition & Food Research·Daniela RegazzoRachel Boutrou
Jan 10, 2013·Journal of Agricultural and Food Chemistry·Satyanarayana Bejjani, Jianping Wu
Aug 14, 2013·Journal of Agricultural and Food Chemistry·Huijuan ZhangWallace Yokoyama
Sep 4, 2015·Journal of Agricultural and Food Chemistry·Long DingJingbo Liu
Mar 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Anna IwaniakMałgorzata Darewicz

❮ Previous
Next ❯

Citations

Feb 23, 2021·Advanced Drug Delivery Reviews·Joël BrunnerGerrit Borchard
Feb 26, 2021·Journal of Agricultural and Food Chemistry·Ritian JinNing Liu

❮ Previous
Next ❯

Software Mentioned

Scan
MS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.