Transepithelial transport of flavanone in intestinal Caco-2 cell monolayers

Biochemical and Biophysical Research Communications
Shoko Kobayashi, Yutaka Konishi

Abstract

Our recent study [S. Kobayashi, S. Tanabe, M. Sugiyama, Y. Konishi, Transepithelial transport of hesperetin and hesperidin in intestinal Caco-2 cell monolayers, Biochim. Biophys. Acta, 1778 (2008) 33-41] shows that the mechanism of absorption of hesperetin involves both proton-coupled active transport and transcellular passive diffusion. Here, as well as analyzing the cell permeability of hesperetin, we also study the transport of other flavanones, naringenin and eriodictyol, using Caco-2 cell monolayers. Similar to hesperetin mentioned, naringenin and eriodictyol showed proton-coupled polarized transport in apical-to-basolateral direction in non-saturable manner, constant permeation in the apical-to-basolateral direction (J(ap-->bl)) irrespective of the transepithelial electrical resistance (TER), and preferable distribution into the basolateral side after apical loading in the presence of a proton gradient. Furthermore, the proton-coupled J(ap-->bl) of hesperetin, naringenin and eriodictyol, were inhibited by substrates of the monocarboxylic acid transporter (MCT), such as benzoic acid, but not by ferulic acid. In contrast, both benzoic and ferulic acids have no stimulatory effect on J(ap-->bl) of each flavanone by trans-stim...Continue Reading

References

Dec 25, 2002·Biological & Pharmaceutical Bulletin·Georges Da ViolantePhilippe Arnaud
Jun 6, 2003·Bioscience, Biotechnology, and Biochemistry·Yutaka Konishi, Makoto Shimizu
Dec 4, 2003·Bioscience, Biotechnology, and Biochemistry·Yutaka KonishiMakoto Shimizu
Apr 29, 2004·Journal of Agricultural and Food Chemistry·Yutaka Konishi, Shoko Kobayashi
Feb 3, 2005·Journal of Agricultural and Food Chemistry·Yutaka Konishi
Jun 9, 2005·Journal of Agricultural and Food Chemistry·Yutaka KonishiEiji Yoshioka
Jun 23, 2005·Journal of Agricultural and Food Chemistry·Adrian A FrankeLaurie J Custer
Jul 12, 2005·Biochimica Et Biophysica Acta·Yutaka Konishi
Dec 22, 2005·Journal of Agricultural and Food Chemistry·Yutaka KonishiEiji Yoshioka
May 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Viktoriya ParoderNancy Carrasco
Aug 24, 2006·Bioscience, Biotechnology, and Biochemistry·Hirohito WatanabeYutaka Konishi
Mar 21, 2007·The British Journal of Nutrition·Claudio GardanaMarisa Porrini
Nov 3, 2007·The Journal of Pharmacy and Pharmacology·Chang-Koo ShimHyo-Kyung Han
Nov 21, 2007·Biochimica Et Biophysica Acta·Shoko KobayashiYutaka Konishi

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Citations

Mar 25, 2011·Critical Reviews in Food Science and Nutrition·R GonzálezF Sánchez de Medina
Dec 14, 2011·The Journal of Pharmacy and Pharmacology·Shoko KobayashiTakuo Ogihara
Aug 2, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Chenlin ShenJun Li
Aug 19, 2016·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Ruonan ChenJun Li
Feb 6, 2019·Nutrients·Sheila Estruel-AmadesMariona Camps-Bossacoma
May 15, 2010·Molecular Nutrition & Food Research·Iris HasslauerPeter Schreier
Aug 29, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Synia HaubIna Bergheim
May 8, 2010·Archives of Biochemistry and Biophysics·Julián Londoño-LondoñoTânia Creczynski-Pasa

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