PMID: 9420040Jan 7, 1998Paper

Transepithelial transport of the fluoroquinolone ciprofloxacin by human airway epithelial Calu-3 cells

Antimicrobial Agents and Chemotherapy
M CavetN L Simmons

Abstract

Although fluoroquinolone antibiotics such as ciprofloxacin are able to gain access to lung tissue and both pleural and bronchial secretions, the characteristics of transport and cellular uptake of ciprofloxacin in human epithelial lung tissue remain obscure. We have chosen human airway epithelial (Calu-3) cells, reconstituted as functional epithelial layers grown on permeable filter supports, as a model with which to assess both transepithelial transport and cellular uptake of ciprofloxacin. Transepithelial ciprofloxacin fluxes in absorptive (apical-to-basal) and secretory (basal-to-apical) directions were similar throughout the concentration range studied (1.0 microM to 3.0 mM). Transepithelial mannitol fluxes measured concurrently were substantially smaller than ciprofloxacin fluxes in Calu-3 epithelia, suggesting the existence of a mediated transcellular route in addition to a paracellular route for transepithelial permeation. Apical-to-basal ciprofloxacin flux (at 10 microM) was inhibited by a 100-fold excess of unlabelled norfloxacin, enoxacin, and ofloxacin, while secretory flux was unaffected. Cellular uptake of ciprofloxacin, determined as a cell/medium ratio, was greater from the basal compartment (2.7-fold) than apica...Continue Reading

References

Sep 1, 1992·The Journal of Antimicrobial Chemotherapy·D Honeybourne, D R Baldwin
Oct 1, 1991·Antimicrobial Agents and Chemotherapy·D E NixJ J Schentag
Oct 1, 1991·Antimicrobial Agents and Chemotherapy·D E NixJ J Schentag
Feb 1, 1991·Antimicrobial Agents and Chemotherapy·F SerourS A Berger
Mar 1, 1990·Diagnostic Microbiology and Infectious Disease·T Bergan
Jun 1, 1990·Antimicrobial Agents and Chemotherapy·D R BaldwinR Wise
Feb 1, 1988·The Journal of Antimicrobial Chemotherapy·W J WijnandsC L van Herwaarden
Jul 1, 1994·American Journal of Respiratory and Critical Care Medicine·R C Boucher
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
Jan 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M KondoJ H Widdicombe
Mar 1, 1993·British Journal of Pharmacology·N M GriffithsN L Simmons

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Citations

May 27, 2011·Pharmaceutical Research·Hui Xin OngPaul Michael Young
Jan 9, 2013·Expert Opinion on Drug Metabolism & Toxicology·Xin LiuMichael S Roberts
Jun 26, 2014·Experimental Biology and Medicine·Joan E NicholsJoaquin Cortiella
Jan 26, 2007·Pharmaceutical Research·Cécile MénezGillian Barratt
Jun 30, 2010·Infection and Immunity·Thomas C SutherlandChristoph M Tang
Jun 5, 2013·Expert Opinion on Drug Delivery·Hui Xin OngPaul M Young
Jun 22, 2005·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·A SteimerC-M Lehr
Nov 22, 2005·Infection and Immunity·Raquel Garcia-MedinaSteven L Brody
Jul 27, 2012·Pharmaceutical Research·Hui Xin OngPaul M Young
Jun 30, 2000·Biochemical Pharmacology·F Van BambekeP M Tulkens
Dec 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S DimovaP Augustijns
Jun 9, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ben Forbes, Carsten Ehrhardt
Jun 28, 2013·Molecular Pharmaceutics·Hui Xin OngPaul M Young
Dec 2, 2009·Journal of Pharmaceutical Sciences·Cynthia Bosquillon
Jul 14, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Paulo PaixãoJosé A G Morais

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