Transepithelial water permeability in microperfused distal airways. Evidence for channel-mediated water transport

The Journal of Clinical Investigation
H G FolkessonA S Verkman

Abstract

Water movement across the airway epithelium is important for regulation of the volume and composition of airspace fluid. A novel approach is reported here to measure osmotic and diffusional water permeability in intact airways. Small airways (100-200 microns diameter, 1-2 mm length) from guinea pig lung were microdissected and perfused in vitro using concentric glass holding and perfusion pipettes. For measurement of osmotic water permeability (Pf), the airway lumen was perfused wit PBS (300 mOsM) containing a membrane impermeable fluorophore, fluorescein sulfonate (FS), and the airway was bathed in solutions of specified osmolalities. Pf determination was based on the changes in FS fluorescence at the distal end of the airway resulting from transepithelial water transport. Pf was 4-5 x 10(-3) cm/s at 23 degrees C and independent of lumen flow rate (10-100 nl/min) and the magnitude and direction of the osmotic gradient (bath osmolality 50-600 mOsM). Temperature dependence measurements gave an activation energy of 4.4 kcal/mol (15-37 degrees C). Pf was not altered by 0.3 mM HgCl2 or 50 microM forskolin, but was increased to 31 x 10(-3) cm/s by 100 micrograms/ml amphotericin B, indicating that osmosis is not limited by unstirred ...Continue Reading

References

Aug 1, 1991·The Journal of Clinical Investigation·M KuwaharaA S Verkman
Dec 1, 1990·The American Review of Respiratory Disease·M A Matthay, J P Wiener-Kronish
Feb 1, 1987·The Journal of Clinical Investigation·Y BerthiaumeM A Matthay
Jan 1, 1970·Respiration Physiology·P T MacklemJ C Hogg
Dec 15, 1993·Biochemical and Biophysical Research Communications·T MaA S Verkman
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·H G FolkessonA S Verkman
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M EchevarriaG Frindt
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A FrigeriA S Verkman
Jul 1, 1994·American Journal of Respiratory and Critical Care Medicine·R C Boucher
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S NielsenP Agre
Nov 1, 1995·The Journal of Membrane Biology·A S Verkman

❮ Previous
Next ❯

Citations

Sep 20, 2005·Pflügers Archiv : European journal of physiology·Peter Steen PedersenOle Frederiksen
Oct 18, 2006·Pflügers Archiv : European journal of physiology·Peter Steen PedersenOle Frederiksen
Feb 1, 2000·Molecular Medicine Today·L S KingP Agre
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·L G DobbsA S Verkman
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·J ZabnerM J Welsh
May 2, 2001·The Journal of General Physiology·J S Landry, D H Eidelman
Jan 5, 2002·The Journal of General Physiology·Ray A CaldwellPierre M Barker
Jan 30, 2002·The Journal of General Physiology·X FangM A Matthay
Oct 15, 2003·The Journal of General Physiology·Yuanlin SongA S Verkman
Dec 30, 1999·American Journal of Respiratory Cell and Molecular Biology·L S KingP Agre
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·S M KredaS E Gabriel
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·L S King, P Agre
Oct 3, 2002·American Journal of Respiratory Cell and Molecular Biology·Sabine BlouquitThierry Chinet
May 17, 2000·The Journal of Clinical Investigation·J W Hanrahan
Sep 1, 1997·The Journal of Clinical Investigation·E P CarterA S Verkman
Aug 26, 1998·The Journal of Clinical Investigation·A FrigeriM Svelto
Sep 17, 1998·The Journal of Clinical Investigation·H MatsuiR C Boucher
Feb 18, 1999·The Journal of Clinical Investigation·C BaiA S Verkman
May 17, 2000·The Journal of Clinical Investigation·H MatsuiR C Boucher
Jan 5, 2000·The Journal of Clinical Investigation·T MaA S Verkman
Sep 21, 2004·Respiratory Research·Lakshmi DurairajJoseph Zabner
Dec 18, 2010·Revue des maladies respiratoires·J MerrienP Chanez
Jun 17, 2008·The Journal of Allergy and Clinical Immunology·Sandra D Anderson, Pascale Kippelen
Apr 17, 2007·Respiratory Physiology & Neurobiology·Sabine Blouquit-Laye, Thierry Chinet
Feb 4, 1999·Biophysical Journal·J E PhillipsD B Yeates
Nov 5, 2016·American Journal of Respiratory Cell and Molecular Biology·Hanna SchmidtOliver H Wittekindt
Mar 21, 2007·Respiratory Physiology & Neurobiology·A S Verkman
Nov 9, 2005·The Journal of Veterinary Medical Science·Seishi MaedaMakoto Seki
Jul 11, 2006·The Journal of Biological Chemistry·Marc H LevinA S Verkman
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Ai-Yu GongNicholas F LaRusso
Jun 28, 2002·Physiological Reviews·Michael A MatthayChristine Clerici
Sep 4, 2015·Physiological Reviews·Jonathan H Widdicombe, Jeffrey J Wine
Jun 3, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·B ResslerR D Kamm
Nov 7, 2018·Pflügers Archiv : European journal of physiology·Oliver H Wittekindt, Paul Dietl
Jul 18, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jonathan H Widdicombe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.