Transesterification of oil mixtures catalyzed by microencapsulated cutinase in reversed micelles

Biotechnology Letters
Sara M BadenesJoaquim M S Cabral

Abstract

Recombinant cutinase from Fusarium solani pisi was used to catalyze the transesterification reaction between a mixture of triglycerides (oils) and methanol in reversed micelles of bis(2-ethylhexyl) sodium sulfosuccinate (AOT) in isooctane for the purposes of producing biodiesel. The use of a bi-phase lipase-catalyzed system brings advantages in terms of catalyst re-use and the control of water activity in the medium and around the enzyme micro-environment. Small-scale batch studies were performed to study the influence of the initial enzyme and alcohol concentrations, and the substrates molar ratio. Conversions in excess of 75 were obtained with reaction times under 24 h, which makes this enzymatic process highly competitive when compared to similar lipase catalyzed reactions for biodiesel production using methanol.

References

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Citations

Mar 8, 2013·Protein Engineering, Design & Selection : PEDS·Iuan-Sheau ChinFarah Diba Abu Bakar
Jan 13, 2012·Applied Biochemistry and Biotechnology·Denise Castro-OchoaAmelia Farrés
Jul 9, 2011·Bioprocess and Biosystems Engineering·Sara M BadenesJoaquim M S Cabral
Nov 13, 2015·Biotechnology Progress·Zhao XiaomanCavaco-Paulo Artur
May 20, 2015·Applied Microbiology and Biotechnology·Antti Nyyssölä
Sep 24, 2013·Biotechnology Advances·Sheng ChenJing Wu
Jan 27, 2017·Applied Biochemistry and Biotechnology·Eva Bermúdez-GarcíaAmelia Farrés
Feb 4, 2011·Biotechnology and Bioengineering·Sara M BadenesJoaquim M S Cabral

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