PMID: 8962124Dec 10, 1996Paper

Transfected Drosophila cells as a probe for defining the minimal requirements for stimulating unprimed CD8+ T cells

Proceedings of the National Academy of Sciences of the United States of America
Z CaiJ Sprent

Abstract

Stimulation of naive T cells by antigen-presenting cells (APC) is thought to involve two qualitatively different signals: signal one results from T-cell receptor (TCR) recognition of antigenic peptides bound to major histocompatibility complex (MHC) molecules, whereas signal two reflects contact with one or more costimulatory molecules. The requirements for stimulating naive T cells were studied with MHC class I-restricted CD8+ T cells from a T-cell receptor transgenic line, with defined peptides as antigen and transfected Drosophila cells as APC. Three main findings are reported. First, stimulation of naive T cells via signal one alone (MHC plus peptide) was essentially nonimmunogenic; thus T cells cultured with peptides presented by MHC class I-transfected Drosophila APC lacking costimulatory molecules showed little or no change in their surface phenotype. Second, cotransfection of two costimulatory molecules, B7-1 and intercellular adhesion molecule 1 (ICAM-1), converted class I+ Drosophila cells to potent APC capable of inducing strong T-proliferative responses and cytokine (interleukin 2) production. Third, B7-1 and ICAM-1 acted synergistically, indicating that signal two is complex; synergy between B7-1 and ICAM-1 varied ...Continue Reading

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Citations

Aug 17, 2000·Springer Seminars in Immunopathology·S SunJ Sprent
Jun 4, 2005·Blood Cells, Molecules & Diseases·Jonathan Sprent
Dec 5, 1998·Journal of Immunological Methods·B K ChoD M Kranz
Jun 24, 1998·The Journal of Experimental Medicine·O S Targoni, P V Lehmann
Aug 10, 2010·The Cancer Journal·Cameron J Turtle, Stanley R Riddell
Oct 31, 1998·Immunological Reviews·P V LehmannT G Forsthuber
Apr 20, 2004·PLoS Biology·Frances CrawfordJohn W Kappler
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Inkyu Hwang, DaLim Ki
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·C R LukschS R Webb
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Inkyu HwangJonathan Sprent
Feb 17, 1997·The Journal of Experimental Medicine·Z CaiJ Sprent
Jun 10, 2006·Hematology/oncology Clinics of North America·Cassian Yee
May 12, 2009·Immunological Reviews·Mandy L Ford, Christian P Larsen
Mar 3, 2010·Immunological Reviews·Kang Liu, Michel C Nussenzweig
Dec 17, 2014·Immunity, Inflammation and Disease·Christian SchützMathias Oelke
Jan 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M R Nicolls, R G Gill
May 28, 2011·Seminars in Immunology·Nina PilatThomas Wekerle
Oct 28, 2008·Journal of Immunological Methods·Esben G W SchmidtMogens H Claesson
Jun 10, 2010·PloS One·John R Harp, Thandi M Onami
Oct 4, 2006·Immunobiology·Clenton ColemanJames E Riggs
Feb 15, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Malcolm S MitchellJune Kan-Mitchell
Aug 24, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jean-Jacques GovalLaurence de Leval
Mar 15, 2001·Nature Immunology·P MarrackJ Kappler
Apr 3, 2004·Nature Biotechnology·Jiyun V KimMichel Sadelain
Jan 6, 2001·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·D J NaisbittB K Park

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