Transfected neu oncogene induces human prostate cancer metastasis

The Prostate
H Y ZhauL W Chung

Abstract

c-erb B2/neu has been demonstrated to be a transforming oncogene in both rodent and human prostatic epithelial cells. To understand the potential role of neu in human prostatic cancer progression, we used a transfer procedure to determine whether neu amplification/overexpression leads to increased tumor growth and metastasis. We chose an androgen-independent human prostatic epithelial cell line, PC-3, as the target for gene transfer. PC-3 cells were cotransfected with pSVneu-T (a point-mutated rat neu oncogene construct) and pSV2neo, and single-cell cloned. Fifty cell clones were isolated and characterized, of which two neu-transfected clones (N17 and N35) and a neo control clone (C32) were studied extensively with respect to neu gene integration, levels of neu mRNA and protein expression, anchorage-independent growth, and tumorigenic and metastatic potential. Results showed that: 1) Clone N35 contained 70 copies of the neu oncogene and a high level of neu mRNA transcripts. It acquired increased anchorage-independent growth potential in vitro and increased tumorigenicity in vivo. 2) Clone N17 contained 10 copies of the neu oncogene and a low level of neu mRNA transcripts. It did not acquire additional capability for anchorage-i...Continue Reading

References

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Citations

Jan 9, 2004·Reproductive Biology and Endocrinology : RB&E·Delisha A StewartRobert A Sikes
Apr 23, 2002·The Prostate·Sarah N SalmE Lynette Wilson
Dec 31, 1997·Pathology International·N KonishiY Hiasa
Jun 19, 2012·Cancer Metastasis Reviews·Frank J Lowery, Dihua Yu
Feb 27, 1999·Endocrine Reviews·E RuijterJ Schalken
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Alexander FossåAasmund Berner
Aug 3, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Senthilmurugan RamalingamVincent C O Njar

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