Transfection efficiency of lipoplexes for site-directed delivery

Journal of Liposome Research
Reint K JellemaPeter M Frederik

Abstract

Targeted gene delivery is a promising strategy to cure disease on its basic level at the site of interest. The ultrastructure, internalization, and transfection efficiency of lipoplexes was investigated. We found that at a charge ratio (rho) of 4.0 lipoplexes had optimum characteristics for gene delivery in vitro. To decrease the size of lipoplexes, we used a method of continuous-flow microfluidics. PEGylation of lipoplexes did not hinder internalization, but was found to hamper transfection. To discriminate between uptake and transfection efficiency of lipoplexes, we used fluorescence-based approaches: microscopy and FACS. To this end, GFP plasmid was labeled with Alexa 594, and, in parallel experiments, GFP plasmid was combined with rhodamine-labeled lipid. Our studies confirm that cellular uptake does not imply transfection efficiency, and that hurdles in cellular processing have to be taken before targeted gene delivery becomes an established therapeutic option.

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·E WagnerM L Birnstiel
Jul 8, 1992·Biochimica Et Biophysica Acta·D NeedhamD D Lasic
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J P LeonettiB Lebleu
Jan 26, 1989·Nature·P L Felgner, G M Ringold
Aug 11, 1995·The Journal of Biological Chemistry·J ZabnerM J Welsh
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·M E DowtyJ A Wolff
May 4, 1995·Biochimica Et Biophysica Acta·H FarhoodL Huang
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·O Zelphati, F C Szoka
May 1, 1997·Nature·D D Lasic
Jul 1, 1997·Nature Biotechnology·N S TempletonG N Pavlakis
Jun 23, 1998·Biophysical Journal·I Rouzina, V A Bloomfield
Feb 27, 1999·Biochemical Society Transactions·R A Laskey
Jul 17, 1999·Trends in Cell Biology·B Talcott, M S Moore
Jan 15, 2000·The Journal of Biological Chemistry·G L LukacsA S Verkman
Mar 4, 2000·Advanced Drug Delivery Reviews·R WattiauxM Jadot
Dec 7, 2000·Cell·D R KlopfensteinS L Rogers
Feb 21, 2002·Molecular Biology of the Cell·Nelly Panté, Michael Kann
Jul 12, 2002·Current Gene Therapy·Delphine Lechardeur, Gergely L Lukacs
Feb 22, 2005·Methods in Enzymology·Peter M Frederik, D H W Hubert
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Greg F WalkerErnst Wagner
Sep 16, 2006·Human Gene Therapy·Delphine Lechardeur, Gergely L Lukacs
Jan 20, 2007·Biochemical Society Transactions·D HoekstraI Zuhorn
Feb 13, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Richard N CohenFrancis C Szoka

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Citations

Jul 3, 2013·Colloids and Surfaces. B, Biointerfaces·Tiago Albertini BalbinoLucimara Gaziola de la Torre

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