Transfection of C6 glioma cells with the bax gene and increased sensitivity to treatment with cytosine arabinoside

Journal of Neurosurgery
M A VogelbaumK M Rich

Abstract

Genes known to be involved in the regulation of apoptosis include members of the bcl-2 gene family, such as inhibitors of apoptosis (bcl-2 and bcl-xl) and promoters of apoptosis (bax). The authors investigated a potential approach for the treatment of malignant gliomas by using a gene transfection technique to manipulate the level of an intracellular protein involved in the control of apoptosis. The authors transfected the murine bax gene, which had been cloned into a mammalian expression vector, into the C6 rat glioma cell line. Overexpression of the bax gene resulted in a decreased growth rate (average doubling time of 32.96 hours compared with 22.49 hours for untransfected C6, and 23.11 hours for clones transfected with pcDNA3 only), which may be caused, in part, by an increased rate of spontaneous apoptosis (0.77 +/- 0.15% compared with 0.42 +/- 0.08% for the vector-only transfected C6 cell line; p = 0.038, two-tailed Student's t-test). Treatment with 1 microM cytosine arabinoside (ara-C) resulted in significantly more cells undergoing apoptosis in the cell line overexpressing bax than in the vector-only control cell line (23.57 +/- 2.6% compared with 5.3 +/- 0.7% terminal deoxynucleotidyl transferase-mediated biotinylated-...Continue Reading

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Citations

Feb 2, 2000·Reviews in Medical Virology·J M MarkertR J Whitley
May 12, 2004·Nature Reviews. Drug Discovery·Jeremy N Rich, Darell D Bigner
Feb 27, 2008·Journal of Neurovirology·Fuwang PengShilpa Buch
May 18, 1999·Current Opinion in Oncology·A Weyerbrock, E H Oldfield
Aug 28, 2010·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Jose Luis JiménezMaría Angeles Muñoz-Fernández
Sep 2, 1999·Journal of Neurosurgery·M A VogelbaumK M Rich
Mar 11, 2003·Technology in Cancer Research & Treatment·Daila S GridleyIstvan Fodor
Dec 12, 2018·Neurosurgery·Esther B DupépéBeverly C Walters
Nov 5, 1999·Neurosurgery·F F LangP J Tofilon

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Methods Mentioned

BETA
transfection
electrophoresis
FACS

Software Mentioned

MCYCLEAV
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