Transfection of Capsaspora owczarzaki , a close unicellular relative of animals

Development
Helena Parra-AceroIñaki Ruiz-Trillo

Abstract

How animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable ...Continue Reading

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Citations

Sep 1, 2018·Molecular Biology and Evolution·Jade SouthworthMartin Carr
Oct 4, 2018·Molecular Biology of the Cell·David S BoothNicole King
Dec 5, 2020·Development·Iñaki Ruiz-Trillo, Alex de Mendoza
Aug 29, 2020·Current Biology : CB·Helena Parra-AceroIñaki Ruiz-Trillo
May 28, 2021·ELife·Ashley L ArthurMargaret A Titus

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Methods Mentioned

BETA
transfection
transfect
fluorescence microscopy
flow cytometry
confocal microscopy
PCR

Software Mentioned

Fiji Imaging
FlowJo
The MYR Predictor
R Stats Package
NMT
R
Huygens System

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