Transfection of dendritic cells (DCs) with the CIITA gene: increase in immunostimulatory activity of DCs

Cancer Gene Therapy
Angela MärtenI G Schmidt-Wolf

Abstract

Dendritic cells (DCs) are the major antigen-presenting cells. They are able to present tumor antigens to immunologic effector cells. MHC class II molecules on DC surfaces play an important role in priming effector cells against tumor cells and their antigens. The transactivator CIITA (MHC class II transactivator) is a non-DNA-binding transactivator, which regulates the expression of MHC class II, HLA-DM, and invariant chain and behaves as a master controller of constitutive and inducible MHC class II gene activation. Here, we transfected DCs with the CIITA gene using a novel transfection technique. The vector system consisted of a plasmid bound to an adenovirus via poly-L-lysine, which is covalently bound to a UV-irradiated adenovirus. After transfection, expression of MHC class II on DCs increased from 27% to 75% on day 2 after transfection. Transfected DCs were co-cultured with immunologic effector cells. Cytotoxicity of effector cells against tumor cells increased after co-culture with transfected DCs to 63% compared to 15% with effector cells co-cultured with irrelevantly transfected DCs (P=.037). This effect was dependent on the timing and period of co-culture. In conclusion, transfection of DCs led to an increase in antit...Continue Reading

Citations

Dec 14, 2004·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yong YouRong Guo
Mar 29, 2002·British Journal of Haematology·G D Schmidt-Wolf, I G H Schmidt-Wolf
Aug 27, 2004·Genetic Vaccines and Therapy·Srinivas NagarajIngo GH Schmidt-Wolf
May 2, 2002·Cell·Jenny Pan-Yun Ting, John Trowsdale
Jun 27, 2002·British Journal of Cancer·G ReinhardI G H Schmidt-Wolf
Feb 1, 2005·World Journal of Gastroenterology : WJG·Ying-Ren ZhaoChang Lu
Dec 10, 2008·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Ho ParkSang-Hoon Lee

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