Transfection of hypoxia-inducible factor-1 decoy oligodeoxynucleotides suppresses expression of vascular endothelial growth factor in oral squamous cell carcinoma cells

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Mie ImaiYoshihide Mori

Abstract

Vasculature development is thought to be an important aspect in the growth and metastasis of solid tumors. Among the many angiogenic factors produced by tumor cells, vascular endothelial growth factor (VEGF) is considered to play a key role in angiogenic processes. VEGF synthesis is modulated by hypoxia-inducible factor-1 (HIF-1) function within the hypoxic microenvironment of growing cancer tissue. To inhibit HIF-1 activation, oligodeoxynucleotides (ODNs) were synthesized and transferred with either the consensus sequence for HIF-1 binding or a mutated form of this sequence. If we could transfer a large number of ODNs into the cancer cell nucleus, activated HIF-1 might bind to the ODNs, resulting in inhibition of hypoxia-induced VEGF synthesis. We transferred these ODNs into cultured oral squamous cell carcinoma cells (SAS cells) using the hemagglutinating virus of Japan (HVJ)-liposome method. Hypoxia-mediated expression of VEGF by cancer cells was suppressed by transfection of HIF-1 decoy ODNs, but not by mutated HIF-1 decoy ODNs. HIF-1 decoy ODN transfection also inhibited VEGF protein synthesis. These results suggest that transfection with HIF-1 decoy ODNs is effective for regulating tumor growth by reducing VEGF.

References

Apr 4, 1997·Cell·K Guillemin, M A Krasnow
Feb 21, 1998·Nucleic Acids Research·T HeinemeyerN A Kolchanov
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·C K GoldmanD T Curiel
Nov 25, 2005·Current Drug Delivery·Tony Azzam, Abraham J Domb
Feb 18, 2011·Journal of Cellular Biochemistry·Jessica Cassavaugh, Karen M Lounsbury
Mar 16, 2011·Brain Research·Girriso F Benderro, Joseph C Lamanna
Apr 5, 2011·Biochemical and Biophysical Research Communications·Kwanghee RyuYoungjoo Lee

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Citations

Oct 22, 2014·International Journal of Oncology·Xuhong ZhuHaidong Zou

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