Transfection of pancreatic islets using polyvalent DNA-functionalized gold nanoparticles.

Surgery
Jonathan S RinkDixon B Kaufman

Abstract

Transplantation of pancreatic islets is an effective treatment for select patients with type 1 diabetes. Improved cellular therapy results may be realized by altering the gene expression profile of transplanted islets. Current viral and nonviral vectors used to introduce nucleic acids for gene regulation hold promise, but safety and efficacy shortcomings motivate the development of new transfection strategies. Polyvalent gold nanoparticles (AuNPs) densely functionalized with covalently immobilized DNA oligonucleotides (AuNP-DNA) are new single entity transfection and gene regulating agents (ie, not requiring lipids, polymers, or viral vectors for cell entry) able to enter cells with high efficiency and no evidence of toxicity. We hypothesize that AuNP-DNA conjugates can efficiently transfect pancreatic islets with no impact on viability or functionality, and can function to regulate targeted gene expression. AuNPs were surface-functionalized with control and antisense DNA oligonucleotides. Purified murine and human islets were exposed to AuNP-DNA conjugates for 24 hours. Islet AuNP-DNA uptake, cell viability, and functionality were measured. Furthermore, the ability of antisense AuNP-DNA conjugates to regulate gene expression w...Continue Reading

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Citations

Jul 26, 2013·Therapeutic Delivery·Leena Loomba, Tiziano Scarabelli
Aug 28, 2012·Advanced Drug Delivery Reviews·Marina G DamianoC Shad Thaxton
May 23, 2012·Biochemistry Research International·Yong WangJosé Oberholzer
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Dec 17, 2020·International Journal of Nanomedicine·Sundus Jabeen Amina, Bin Guo

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