Abstract
Transplantation of pancreatic islets is an effective treatment for select patients with type 1 diabetes. Improved cellular therapy results may be realized by altering the gene expression profile of transplanted islets. Current viral and nonviral vectors used to introduce nucleic acids for gene regulation hold promise, but safety and efficacy shortcomings motivate the development of new transfection strategies. Polyvalent gold nanoparticles (AuNPs) densely functionalized with covalently immobilized DNA oligonucleotides (AuNP-DNA) are new single entity transfection and gene regulating agents (ie, not requiring lipids, polymers, or viral vectors for cell entry) able to enter cells with high efficiency and no evidence of toxicity. We hypothesize that AuNP-DNA conjugates can efficiently transfect pancreatic islets with no impact on viability or functionality, and can function to regulate targeted gene expression. AuNPs were surface-functionalized with control and antisense DNA oligonucleotides. Purified murine and human islets were exposed to AuNP-DNA conjugates for 24 hours. Islet AuNP-DNA uptake, cell viability, and functionality were measured. Furthermore, the ability of antisense AuNP-DNA conjugates to regulate gene expression w...Continue Reading
References
Jan 1, 1989·Diabetes·C RicordiD W Scharp
Mar 27, 1995·Transplantation·A M DavalliG C Weir
Aug 22, 1997·Science·R ElghanianC A Mirkin
Apr 2, 1999·Diabetes·G LeibowitzF Levine
Jul 27, 2000·The New England Journal of Medicine·A M ShapiroR V Rajotte
Sep 8, 2000·Science·T A TatonR L Letsinger
Sep 28, 2001·Diabetes·H HeimbergD L Eizirik
Jan 5, 2002·Diabetes·Montserrat BiarnésEduard Montanya
Feb 6, 2003·Journal of the American Chemical Society·Rongchao JinGeorge C Schatz
Feb 8, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ram I MahatoA Osama Gaber
Aug 30, 2003·Surgery·Marshall S BakerDixon B Kaufman
Mar 30, 2004·Diabetes·Nan ZhangHengjiang Dong
Jun 29, 2005·Diabetes·Edmond A RyanA M James Shapiro
May 20, 2006·Science·Nathaniel L RosiChad A Mirkin
May 23, 2006·Pharmacological Reviews·Ajit S Narang, Ram I Mahato
Nov 14, 2007·Nano Letters·David A GiljohannChad A Mirkin
Oct 3, 2008·Molecular Pharmaceutics·Guofeng ChengRam I Mahato
Jan 28, 2009·Journal of the American Chemical Society·David A GiljohannChad A Mirkin
Oct 3, 2009·Cell Transplantation·UNKNOWN CITR Research Group
Citations
Jul 26, 2013·Therapeutic Delivery·Leena Loomba, Tiziano Scarabelli
Aug 28, 2012·Advanced Drug Delivery Reviews·Marina G DamianoC Shad Thaxton
May 23, 2012·Biochemistry Research International·Yong WangJosé Oberholzer
Oct 2, 2012·Journal of the American Chemical Society·Ke ZhangChad A Mirkin
May 21, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sungsook AhnSang Joon Lee
Oct 11, 2011·Surgery·Jonathan S RinkDixon B Kaufman
Jan 7, 2011·Trends in Biotechnology·Chung-Huei K Wang, Suzie H Pun
Jun 11, 2011·Small·Alexander KiegerC Shad Thaxton
Jan 8, 2013·Nano Today·Lara YildirimerAlexander M Seifalian
Jul 26, 2011·Chemical Society Reviews·Audrey Llevot, Didier Astruc
Jul 6, 2016·Lab on a Chip·Pamuditha N SilvaJonathan V Rocheleau
Jun 14, 2011·Nanomaterials·Pooja M TiwariShree R Singh
Mar 14, 2019·The Analyst·Xinxin LiKun Xu
Nov 16, 2018·Recent Patents on Drug Delivery & Formulation·Simpal KumariGyanendra Nath Singh
Feb 28, 2014·Materials·Mosaad A Abdel-WahhabBertrand H Rihn
Nov 20, 2013·Transplantation·Jonathan S RinkDixon B Kaufman
Dec 17, 2020·Chemical Society Reviews·Sabine SzuneritsRabah Boukherroub
Dec 17, 2020·International Journal of Nanomedicine·Sundus Jabeen Amina, Bin Guo