Transfection of PDCD5 effect on the biological behavior of tumor cells and sensitized gastric cancer cells to cisplatin-induced apoptosis.

Digestive Diseases and Sciences
Hui-Yu XuYou-Yong Lu

Abstract

Programmed cell death 5 (PDCD5) expression is reduced in various human tumor cells, and the protein concentration and nuclear translocation of PDCD5 is also observed during tumor cell apoptosis. The purpose of this study was to investigate the differential expression of PDCD5 in six gastric cell lines, and to explore the changes of biological behavior mechanism underlying enhanced apoptosis-inducing effects of cisplatin by PDCD5 over-expression on gastric cancer BGC823 cells. RT-PCR and real-time PCR were used to determine PDCD5 expression. BGC823/PDCD5 cells were assessed the cellular proliferating ability by MTT assay, soft agar cloning experiments and tumorigenicity in nude mice experiments in vivo. The effects of cisplatin in combination with PDCD5 on the proliferation and apoptosis were measured by MTT, Annexin-V-FITC/PI dual labeling and cell cycle analysis, respectively. Immunofluorescence was used to detect co-localization of p53 and PDCD5 protein to explore the mechanism underlying the synergistic therapeutic effect of PDCD5 with cisplatin (5 μg/ml for 24 h). PDCD5 had the highest expression level in the GES1 cell among other cell lines. The growths of BGC823 cells transfected with PDCD5 for six (6th) or 17 (17th) days...Continue Reading

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Citations

Nov 14, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lanlan WangLu Wang
Dec 18, 2013·Apoptosis : an International Journal on Programmed Cell Death·Farhan MurshedJoseph A Fontana
Jan 19, 2016·Biochimica Et Biophysica Acta·Ge LiYingyu Chen
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Feb 7, 2018·Oncology Letters·Penghui LiLihong Zheng
Aug 26, 2018·Journal of Cellular Physiology·Sungmin KwakKyung-Chul Choi
Jun 21, 2018·Cellular & Molecular Immunology·Ke WangQing Ge
Mar 29, 2018·Proceedings of the National Academy of Sciences of the United States of America·Seung-Hyun LeeHo-Geun Yoon
Nov 9, 2016·International Journal of Oncology·Wei WangCheng-Hai Zhao

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis