PMID: 9422547Jan 9, 1998Paper

Transfection of rat ovarian surface epithelium with erb-B2/neu induces transformed phenotypes in vitro and the tumorigenic phenotype in vivo

The American Journal of Pathology
B R DaviesB A Ponder

Abstract

The neu/cerb-B2 gene is frequently amplified and/or overexpressed in human epithelial ovarian cancers. We have established an inbred animal model for ovarian cancer that mimics aspects of human ovarian cancer by transducing a spontaneously immortalized rat ovarian surface epithelial cell line in culture with ecotropic retroviruses expressing a mutated rat neu/c-erb-B2 oncogene. Transfectants expressing neu at a high level exhibited altered morphology and behavior in two-dimensional and three-dimensional culture in Matrigel, could be cloned in soft agar, and were more invasive through a Matrigel membrane than control transfectants transduced with a similar retrovirus expressing the beta-galactosidase gene. When injected intraperitoneally, neu-expressing transfectants produced highly invasive, rapidly growing tumors that coated the peritoneal cavity and induced ascites formation. Furthermore, neu transfectants could be grown as solid tumors when injected subepithelially into the ovary. The neu-transfected cells also formed tumors when injected subcutaneously into the mammary fat pad, although they grew relatively poorly and often regressed. Transfectants expressing beta-galactosidase failed to produce tumors at any of the sites i...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.