Transfection of the type II TGF-beta receptor into colon cancer cells increases receptor expression, inhibits cell growth, and reduces the malignant phenotype

Annals of Surgery
Sally L D MacKayEdward M Copeland

Abstract

To determine if transfection of SW48 colon cancer cells with the type II transforming growth factor-beta (TGF-beta) receptor restores growth inhibition and reverses the in vitro and in vivo malignant phenotype. The authors have previously shown that SW48 colon cancer cells that are replication error positive in both alleles lack functional cell surface TGF-beta type I (RI) and type II (RII) receptors and are insensitive to TGF-beta1-induced growth inhibition. SW48 cells were stably transfected with the cDNA for the normal type II TGF-beta receptor (RII). Once transfected, the cells were evaluated for in vitro phenotypic changes and in vivo changes in tumor growth. Denaturing sequencing gel electrophoresis of the reverse transcriptase-polymerase chain reaction product from SW48 cells revealed that the RII coding sequence contained a single base deletion mutation. When these cells were transfected with normal RII cDNA, Northern and Western blot analyses revealed increased levels of RII mRNA and protein. Affinity labeling techniques revealed that RII-transfected SW48 cells produced functional RI and RII protein. Transfection of SW48 cells also led to changes in cell phenotype, as shown by inhibition of both in vitro growth rate an...Continue Reading

References

Jan 1, 1990·Annals of the New York Academy of Sciences·J MassaguéJ L Andres
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Jan 1, 1990·Annals of the New York Academy of Sciences·R J CoffeyJ A Barnard
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A BarnardH L Moses
Jan 1, 1987·Methods in Enzymology·J Massagué
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K SellheyerD R Roop
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·D F PierceH L Moses
Aug 4, 1994·Nature·J L WranaJ Massagué
Jun 1, 1996·Cytokine & Growth Factor Reviews·S D Markowitz, A B Roberts

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Citations

Mar 29, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ganesan Ramamoorthi, Nageswaran Sivalingam
Nov 6, 2008·International Journal of Colorectal Disease·Janindra WarusavitarneMargaret Schnitzler
Mar 29, 2001·Thyroid : Official Journal of the American Thyroid Association·K M SchulteH D Röher
Dec 22, 1999·Journal of the National Cancer Institute·H GobbiD L Page
Apr 25, 2003·International Journal of Cancer. Journal International Du Cancer·Philipp BeckhoveVolker Schirrmacher
Dec 28, 1999·Microbes and Infection·M Reiss

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