Transfer cells mediate nitrate uptake to control root nodule symbiosis.

Nature Plants
Qi WangZhaosheng Kong

Abstract

Root nodule symbiosis enables nitrogen fixation in legumes and, therefore, improves crop production for sustainable agriculture1,2. Environmental nitrate levels affect nodulation and nitrogen fixation, but the mechanisms by which legume plants modulate nitrate uptake to regulate nodule symbiosis remain unclear1. Here, we identify a member of the Medicago truncatula nitrate peptide family (NPF), NPF7.6, which is expressed specifically in the nodule vasculature. NPF7.6 localizes to the plasma membrane of nodule transfer cells (NTCs), where it functions as a high-affinity nitrate transporter. Transfer cells show characteristic wall ingrowths that enhance the capacity for membrane transport at the apoplasmic-symplasmic interface between the vasculature and surrounding tissues3. Importantly, knockout of NPF7.6 using CRISPR-Cas9 resulted in developmental defects of the nodule vasculature, with excessive expansion of NTC plasma membranes. npf7.6 nodules showed severely compromised nitrate responsiveness caused by an attenuated ability to transport nitrate. Moreover, npf7.6 nodules exhibited disturbed nitric oxide homeostasis and a notable decrease in nitrogenase activity. Our findings indicate that NPF7.6 has been co-opted into a regu...Continue Reading

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Citations

Dec 21, 2020·Physiologia Plantarum·Yuming SunShiwei Guo
Dec 17, 2020·Journal of Integrative Plant Biology·Baocai ZhangYihua Zhou
Jul 6, 2021·Frontiers in Plant Science·Ylenia VittozziMaurizio Chiurazzi
Jul 10, 2021·Frontiers in Plant Science·Yanlin Ma, Rujin Chen
Sep 16, 2021·The EMBO Journal·Qiying XiaoJeremy D Murray

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Methods Mentioned

BETA
transgenic
scanning electron microscopy
transmission electron microscopy
PCR
RNA-seq

Software Mentioned

MapMan
Origin 2018
GraphPad Prism
Image J
XTension
Imaris
Excel
HISAT
R

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