PMID: 1199022Jan 1, 1975Paper

Transfer factor preparations: some physico-chemical and biological properties

Vox Sanguinis
J F Reymond, P J Grob

Abstract

Some biological and physico-chemical properties were tested in 50 lots of transfer factor (TF). Each lot contained the extracts of a pool of 20 buffy coats, each originating from a 400 ml blood donation of a healthy individual. The optical densities at 260 and 280 nm were within a narrow range in all TF lots. The ratio 260/280 was 2.56-3.02. One unit TF (defined as the extract of two buffy coats (corresponding to 1-2 X 10(9) leucocytes) contained 36.58 +/- 3.77 mg of peptides as measured by the Lowry method and 123.6 +/- 4.5 mg ribose as estimated by the orcinol method. Cytotoxicity of TF lots was tested by measurement of chromium release of PHA-stimulated lymphocytes. Cytotoxicity was above 10% in 20 of 27 TF lots tested, most of which were produced by an Amicon ultrafiltration cell. Pyrogenicity was present in 8 of 42 TF lots produced by Amicon filters, but only in 1 of 8 lots produced by Sartorius filters. Sephadex G-10 chromatograms of TF gave characteristic patterns yielding 8 peaks. Sephadex G-25 chromatograms reproducibly showed 5 peaks. The distribution of osmotic activity, peptides and ribose in respect of the chromatographic fractions is given.

References

Jan 1, 1975·Vox Sanguinis·A BastenD S Nelson
Feb 1, 1975·European Journal of Clinical Investigation·P J Grob
Oct 13, 1973·Lancet·A A GottliebM Lopez
Oct 1, 1974·The Journal of Clinical Investigation·K S ZuckermanA F LoBuglio
Oct 1, 1974·The Journal of Obstetrics and Gynaecology of the British Commonwealth·P J Grob, B Wüthrich
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B DupontR A Good
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M S AscherH S Lawrence
Jan 1, 1974·Cleveland Clinic Quarterly·F B PriceB Barna
Nov 23, 1972·The New England Journal of Medicine·W E BullockM W Brandriss
Jan 1, 1973·Annual Review of Medicine·A S LevinH H Fudenberg
Jan 1, 1974·Oncology·C H Kirkpatrick, J I Gallin
Sep 1, 1974·The Journal of Clinical Investigation·A CatanzaroK M Moser
Dec 1, 1972·The Journal of Clinical Investigation·L E SpitlerR A Gatti
Mar 2, 1973·Deutsche medizinische Wochenschrift·F BläkerK H Schulz
Aug 1, 1973·Annals of Internal Medicine·M E Whitcomb, R E Rocklin
Nov 1, 1973·Cellular Immunology·J A NeidhartA F LoBuglio
Mar 1, 1974·Annals of Internal Medicine·C H Kirkpatrick, T K Smith
Aug 1, 1974·The Journal of Allergy and Clinical Immunology·F Perlman
Jan 1, 1967·International Archives of Allergy and Applied Immunology·M P Arala-ChavesJ F Heremans
Jan 1, 1960·The Journal of Clinical Investigation·H S LAWRENCEW S TILLETT
Aug 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·H S LAWRENCE

❮ Previous
Next ❯

Citations

Feb 1, 1992·Molecular Immunology·S J Rozzo, C H Kirkpatrick
Jan 1, 1975·Vox Sanguinis·A BastenD S Nelson
Jul 20, 1990·Journal of Chromatography·P C MontecucchiG Sirchia
Jan 1, 1978·Scandinavian Journal of Infectious Diseases·R WinsnesM I Degré
Nov 1, 1977·Acta paediatrica Scandinavica·F K PedersenM Thomsen
Mar 1, 1977·Clinical Immunology and Immunopathology·G B WilsonH H Fudenberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.