PMID: 6979044Mar 1, 1982Paper

Transfer of an antigen-specific immediate hypersensitivity-like reaction with an antigen-binding factor produced by T cells

Proceedings of the National Academy of Sciences of the United States of America
W PtakR K Gershon

Abstract

The fact that T cell-dependent activation of mast cells occurs in delayed-type hypersensitivity led us to investigate whether a T cell product could mimic some of the functions of IgE. We report that 24- or 48-hr cultures of T cells from mice immunized optimally for delayed-type hypersensitivity resulted in release of an antigen-binding factor that transferred the ability to elicit an antigen-specific immediate hypersensitivity-like skin reaction in normal recipients. The responsible factor was concentrated and purified by affinity chromatography on antigen columns and was distinguished from immunoglobulin by several criteria: (i) it was released by purified T cells (anti-immunoglobulin plate depletion of B cells); (ii) it expressed no known antigenic markers of immunoglobulins (enzyme-linked immunosorbant direct binding assay); (iii) it had a molecular weight of 70,000 or less (sucrose gradient ultracentrifugation); and (iv) it had serological markers associated with antigen-specific T cell factors from other experimental systems. We suggest that, at sites of delayed-type hypersensitivity, antigen-reactive T cells may release antigen-specific factors that lead to mast cell activation and release of vasoactive amines, which is ...Continue Reading

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L J Wysocki, V L Sato
Sep 1, 1975·The Journal of Experimental Medicine·R K GershonM D Gershon
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R W RosensteinR K Gershon
Nov 1, 1980·The Journal of Experimental Medicine·P W AskenaseR K Gershon
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R E ConeR K Gershon

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Citations

Apr 1, 1985·Molecular Immunology·R E Cone, K D Beaman
Jul 1, 1996·Immunology Today·R E Cone, A Malley
Aug 1, 1994·International Journal of Immunopharmacology·N MurrayY Bonhomme
Mar 1, 1983·The Journal of Experimental Medicine·P W AskenaseW Ptak
May 1, 1983·The Journal of Experimental Medicine·H van LoverenP W Askenase
Dec 1, 1988·The Journal of Clinical Investigation·R AlamM A Lett-Brown
Feb 19, 2015·Archivum Immunologiae Et Therapiae Experimentalis·Włodzimierz PtakKrzysztof Bryniarski
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vipin PaliwalPhilip W Askenase
Jan 1, 1988·Scandinavian Journal of Rheumatology. Supplement·H Prydz

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