PMID: 9530517Apr 8, 1998Paper

Transfer of cholesterol from macrophages to lymphocytes in culture

Biochemistry and Molecular Biology International
P I de Bittencourt Júnior, Rui Curi

Abstract

A major feature of macrophage metabolism is its capacity to produce and export cholesterol. Several reports have shown that the manipulation of lymphocyte cholesterol content elicits important changes in lymphocyte proliferation. These findings lead to an inquiry as to whether macrophage-derived cholesterol released into the lymphocyte surroundings may be transferred to the latter thus affecting lymphocyte function. In this study, cholesterol transfer from macrophages to lymphocytes was examined in vitro using rat cells in culture. The findings indicate that there may be a significant transfer of cholesterol from [4-14C]cholesterol labeled resident peritoneal macrophages to mesenteric lymph node resting lymphocytes (up to 173.9 +/- 2.7 pmol/10(7) lymphocytes/10(7) macrophages when co-cultivated for 48 h), in a lipoprotein-dependent manner. This represents the mass transfer of ca. 17 nmoles of cholesterol molecules per 10(7) lymphocytes from 10(7) macrophages (calculated on the basis of specific radioactivity incorporated into macrophages after the pre-labelling period), which suggests that macrophages are capable of replacing the whole lymphocyte cholesterol pool every 21 h. Moreover, an 111%-increase in the total cholesterol c...Continue Reading

Citations

Nov 1, 2001·Developmental and Comparative Immunology·Charles A WestSteven J Mentzer
Nov 19, 2003·Cell Biochemistry and Function·C M PeresR Curi
Apr 29, 2008·Biochemical Pharmacology·Lucila Ludmila Paula GutierrezPaulo Ivo Homem de Bittencourt
Sep 20, 2005·Cell Biochemistry and Function·Carmem Maldonado PeresRui Curi
Jun 3, 2010·Cell Biochemistry and Function·Gleisson A P BritoLuiz C Fernandes
Nov 5, 2002·Cell Biochemistry and Function·Marilena da CostaRui Curi
Feb 27, 2001·Cell Biochemistry and Function·J R GarciaA R Carpinelli
Jan 18, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J GazzolaR Curi

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