Transfer of cobalamin from the cobalamin-binding protein of egg yolk to R binder of human saliva and gastric juice

Gastroenterology
A Del Corral, R Carmel

Abstract

Patients may fail to absorb cobalamin (vitamin B12) bound to food even when they have adequate intrinsic factor to absorb free cobalamin normally. We studied cobalamin transfer from egg yolk cobalamin-binding protein to human saliva and gastric juice as a model of this important first step in cobalamin assimilation. The cobalamin-binding protein of egg yolk eluted with human R binder on Sephadex gel chromatography and bound cobalamin with a comparable affinity, but it did not cross-react with R binder immunologically. Transfer of cobalamin from egg yolk to saliva or gastric juice R binder did not occur at neutral pH. Slight transfer (8%-12% of the 57Co-cobalamin bound to egg yolk) occurred when the saliva was acidified to pH 1.5. This minor transfer by acid was not inhibited by pepstatin A, a pepsin inhibitor. Acidification caused variable transfer to gastric juice R binder (12%-40%) that appeared to be partially due to residual gastric pepsin activity. Adding 1200 U of pepsin per milliliter enhanced cobalamin transfer to saliva or gastric juice R binders (39%-58% transfer). At no time was cobalamin transferred directly to intrinsic factor; R binder-deficient gastric juice failed to accept cobalamin from egg yolk. The transfer ...Continue Reading

Citations

Feb 24, 2007·Digestive Diseases and Sciences·Sebahat OzdemReha Artan
Dec 23, 2003·Alimentary Pharmacology & Therapeutics·L SantarelliA Gasbarrini
May 11, 2005·World Journal of Gastroenterology : WJG·Birol OzerSedat Boyacioglu
Mar 17, 2010·Indian Pediatrics·Mustafa Akcam
Apr 16, 2013·Organic & Biomolecular Chemistry·Mai Thanh Quynh TranRoger Alberto
Aug 1, 1996·Journal of the American Geriatrics Society·D Z van AsseltW H Hoefnagels
Dec 25, 2002·Journal of Pediatric Gastroenterology and Nutrition·Lisa M RogersLindsay H Allen
Feb 1, 1994·Digestive Diseases and Sciences·R CarmelM J Blaser
Mar 5, 2008·Alimentary Pharmacology & Therapeutics·B I HirschowitzJ Mohnen
Oct 17, 2003·World Journal of Gastroenterology : WJG·Javed YakoobShahab Abid
Jan 11, 2007·Digestive Diseases and Sciences·Mustafa AkcamReha Artan
Apr 1, 2019·Journal of Dairy Science·Sergey N FedosovChristian W Heegaard
Dec 1, 1994·Digestive Diseases and Sciences·R Carmel
Apr 29, 2015·Clinics and Practice·Shrikant C RautGirish D Bakhshi

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