Transfer of eukaryotic expression plasmids to mammalian hosts by attenuated Salmonella spp

International Journal of Medical Microbiology : IJMM
S Weiss

Abstract

Transkingdom transfer of DNA from bacteria to other organisms, well established for bacteria, yeast and plants, was recently also extended to mammalian host cells. Attenuated intracellular bacteria or non-pathogenic bacteria equipped with adhesion and invasion properties have been demonstrated to transfer eukaryotic expression plasmids in vitro and in vivo. Here the mucosal application of attenuated Salmonella enterica spp. as DNA carrier for the induction of immune responses towards protein antigens encoded by expression plasmids, their use to complement genetic defects or deliver immunotherapeutic proteins is reviewed. Plasmid transfer has been reported for Salmonella typhimurium, S. typhi and S. choleraesuis so far but clearly other Salmonella strains should be able to transfer expression plasmids as well. Transfer of DNA is effected most likely by bacterial death within the host cell resulting from metabolic attenuation. Since these bacteria remain in the phagocytic vacuole it is unclear how the DNA from such dying bacteria is delivered to the nucleus of infected cells. Nevertheless, the efficiency that has been observed was astonishingly high, reaching close to 100% under certain conditions. Gene transfer in vivo was mainl...Continue Reading

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Citations

Mar 5, 2004·Expert Opinion on Biological Therapy·Holger Loessner, Siegfried Weiss
Nov 25, 2004·Current Opinion in Biotechnology·Chakameh Azimpour TabriziWerner Lubitz
Apr 6, 2007·The Journal of Gene Medicine·Caroline AgorioJose Alejandro Chabalgoity
Nov 30, 2010·Applied Microbiology and Biotechnology·Won Suck YoonYong Keun Park
Nov 19, 2018·Applied Microbiology and Biotechnology·Tingtao ChenHongbo Xin
Jul 4, 2021·Current Opinion in Biotechnology·Dongdong NiuHailing Jin

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