Transfer of genes to chondrocytic cells of the lumbar spine. Proposal for a treatment strategy of spinal disorders by local gene therapy

Spine
P WehlingJ A Reinecke

Abstract

In the current study, chondrocytic cells from bovine intervertebral end plates were cultivated in vitro and modified genetically. The authors intended to perform isolation and cultivation of cells from bovine end plates of the spine. They also intended to show, in principle, the feasibility of introducing exogenous genes into chondrocytic cells from bovine intervertebral end plates by way of retroviral vectors. The involvement of cytokines in the destruction of articular cartilage is established. It appears possible that similar mechanisms may play a role in intervertebral disc degeneration and other spinal disorders. Conventional medication and surgery of intervertebral disc degeneration addresses neither the pathophysiology nor the chronicity of the disease. Therapeutic proteins carry great potential as locally produced drugs after transfer of their cognate genes to the sites of interest. Vertebral end plate tissue was obtained from bovine os coccygis. Chondrocytic cells were isolated and cultured in vitro. The bacterial beta-galactosidase (LacZ) gene and, alternatively, the complementary DNA (DNA copy of the mRNA) of the human interleukin-1 receptor antagonist were introduced into the isolated cells by retrovirus mediated ge...Continue Reading

References

Nov 1, 1992·British Journal of Pharmacology·F Q CunhaS H Ferreira
Apr 1, 1992·DNA and Cell Biology·G BandaraC H Evans
Jun 11, 1992·Nature·A D Miller
Jul 1, 1992·Journal of Neuroimmunology·S RotshenkerV Barak
Nov 1, 1991·British Journal of Pharmacology·F Q CunhaS H Ferreira
Apr 1, 1989·Archives of Biochemistry and Biophysics·S WatanabeC H Evans
Aug 1, 1989·The Journal of Clinical Investigation·D D DeanJ F Woessner
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O Danos, R C Mulligan
Nov 1, 1994·Zeitschrift für Orthopädie und ihre Grenzgebiete·C H EvansJ Reinecke
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G BandaraC H Evans
Jan 1, 1993·Advances in Immunology·W P Arend
Jan 1, 1993·Zeitschrift für Orthopädie und ihre Grenzgebiete·P WehlingC H Evans

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Citations

Jul 26, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Koichi Masuda, Howard S An
Sep 20, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Günther PaesoldNorbert Boos
Nov 14, 2008·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Kotaro NishidaMinoru Doita
May 9, 2012·Advances in Orthopedics·Fadi TaherFederico P Girardi
Nov 1, 2003·The Spine Journal : Official Journal of the North American Spine Society·E H CassinelliJ D Kang
Jun 6, 2009·Journal of Spinal Disorders & Techniques·Daniel R FassettAlexander R Vaccaro
Jan 26, 2007·Expert Opinion on Biological Therapy·Gianluca VadalàJames D Kang
Dec 3, 2014·Advanced Drug Delivery Reviews·S B G BlanquerA A Poot
Sep 29, 2011·The Orthopedic Clinics of North America·Barrett I WoodsJames D Kang
Dec 4, 2003·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Jun LiS Tim Yoon
Nov 27, 2004·Spine·Adam L ShimerJames D Kang
Dec 18, 2007·The Spine Journal : Official Journal of the North American Spine Society·Satoshi SobajimaJames D Kang
Jan 30, 2007·Journal of Magnetic Resonance Imaging : JMRI·S RajasekaranSubramaniam Murugan
Jan 27, 2006·International Journal of Experimental Pathology·Christine L Le MaitreJudith A Hoyland
May 9, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Gladius Lewis
Apr 4, 2006·Injury·Peter V GiannoudisEleftherios Tsirids
Nov 18, 2005·The Spine Journal : Official Journal of the North American Spine Society·Eric A LevicoffJames D Kang
Oct 23, 2002·The Orthopedic Clinics of North America·Chisa HidakaHarvinder S Sandhu
Jan 23, 2003·The Journal of Gene Medicine·Johnny HuardFreddie H Fu
Jan 1, 2012·Stem Cells International·Umile Giuseppe LongoVincenzo Denaro
Jan 18, 2014·Global Spine Journal·Fackson Mwale
Jan 16, 2004·Gene Therapy·S SobajimaJ D Kang
Jun 13, 2017·The Open Orthopaedics Journal·Ibrahim Halil UralSüleyman Özyalçın
Feb 13, 2015·Journal of the Royal Society, Interface·Maria MolinosMário A Barbosa
Nov 22, 2019·Current Pharmaceutical Biotechnology·Songfeng ChenHongjian Liu
Nov 5, 1999·Clinical Orthopaedics and Related Research·C H Evans, P D Robbins
Jul 10, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shirley TangDevina Purmessur
Apr 7, 2020·Neurospine·Yoshiki TakeokaKotaro Nishida
Jun 6, 2008·The Journal of the American Academy of Orthopaedic Surgeons·Mark G HubertJames D Kang
Feb 10, 2021·International Journal of Molecular Sciences·Eun Ji RohInbo Han

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