PMID: 4899853Nov 1, 1969Paper

Transfer of responsiveness to hapten conjugates of poly-L-lysine and of a copolymer of L-glutamic acid and L-lysine to lethally irradiated nonresponder guinea pigs by bone marrow or lymph node and spleen cells from responder guinea pigs

The Journal of Experimental Medicine
J FoersterB BENACERRAF

Abstract

Hartley guinea pigs genetically unresponsive to hapten-PLL (poly-L-lysine) conjugates were lethally irradiated and given allogeneic bone marrow from Hartley responder animals. Many of the animals died of graft versus host disease before their response to 2,4-dinitrophenyl-PLL (DNP-PLL) could be measured. The immune response of the surviving recipient animals was evaluated by anti-DNP antibody production, development of delayed hypersensitivity to DNP-poly-L-lysine, as well as by lymph node cell stimulation in vitro by this antigen. 12 of 14 recipient animals thus treated made an immune response as measured by 2 of the 3 parameters. Strain 13 guinea pigs, genetically unable to respond immunologically to DNP-PLL and to DNP-GL (2,4-dinitrophenyl-L-glutamic acid L-lysine copolymer) were lethally irradiated and given bone marrow from (2 x 13) F(1) responder animals or strain 13 bone marrow and (2 x 13) F(1) lymph node and spleen cells. A high proportion of the animals survived this procedure; no evidence of graft versus host disease was observed. Three of three strain 13 animals irradiated and, given strain 13 bone marrow and (2 x 13) F(1) lymph node and spleen, and then immunized with DNP-PL, made a specific immune response. 7 of 1...Continue Reading

References

Aug 1, 1965·Biochemistry·S F SchlossmanH A Sober

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Citations

Jun 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·L EllmanB Benacerraf
Aug 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·I GreenB Benacerraf
Sep 1, 1970·The Journal of Experimental Medicine·H F DvorakM J Karnovsky
Nov 1, 1971·The Journal of Experimental Medicine·D G Tubergen, J D Feldman
Jan 1, 1978·Immunological Reviews·B Benacerraf, R N Germain
Mar 1, 1970·Angewandte Chemie·E Rüde
Dec 1, 1973·European Journal of Immunology·U WürzburgK Rajewsky
Dec 1, 1973·European Journal of Immunology·I MelchersD C Shreffler

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