Transfer of spermatogenesis-related cDNAs into eel testis germ-somatic cell coculture pellets by electroporation: methods for analysis of gene function

Molecular Reproduction and Development
Chiemi I MiuraTakeshi Miura


Genes encoding spermatogenesis-related substance (eSRSs) show unique expression patterns during spermatogenesis. To analyze their function, we developed a new assay system using gene transfer techniques combined with coculture of the eel germ-somatic cells. First, we investigated the efficacy of in vitro electroporation transfer of gene into germ-somatic cell pellets using green fluorescent protein (GFP) gene. Second, in order to define the function of the eSRSs, we electrophoretically transferred eel spermatogonial stem cell renewal factor (eSRS34) and eel spermatogenesis-preventing substance (eSRS21) genes into germ-somatic cell pellets. Presence of the transferred cDNA was examined by reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, proliferating cells were detected histologically, after labeling with BrdU. Transfer of the eSRS34 gene induced spermatogonial stem cell renewal in the pellets. Moreover, 11-ketotestosterone (11-KT) treatment stimulated the proliferation of spermatogonia, which resulted in the appearance of late type B spermatogonia in the pellets. The proliferation of spermatogonia by 11-KT stimulation was suppressed by transfer of the eSRS21 gene. These results indicate that the transferre...Continue Reading


Dec 1, 1991·Molecular Reproduction and Development·C L BrazolotA M Verrinder Gibbins
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Takeshi MiuraY Nagahama
Feb 11, 1994·Science·M ChalfieD C Prasher
Aug 31, 1999·Development, Growth & Differentiation·Chiemi I MiuraKohei Yamauchi
Oct 21, 1999·Biochemical and Biophysical Research Communications·Takeshi MiuraK Yamauchi
Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
Feb 25, 2000·Molecular and Cellular Biology·Q LinA I Skoultchi
Oct 23, 2001·Genesis : the Journal of Genetics and Development·R Sussman
Jan 17, 2002·Journal of Immunological Methods·Zhi ChenRiitta Lahesmaa
Feb 21, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Nathalie DujardinVeronique Préat
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kayoko KuritaNoriyoshi Sakai


Dec 20, 2015·ACS Applied Materials & Interfaces·Hongfei YuHaiyan Li
Oct 3, 2015·General and Comparative Endocrinology·Frank PfennigHerwig O Gutzeit
Aug 14, 2013·PloS One·Shin KobayashiFumitoshi Ishino
Mar 5, 2011·Annual Review of Analytical Chemistry·Adam Johan Bergren, Richard L McCreery
Dec 28, 2017·Human Reproduction Update·João Pedro Alves-Lopes, Jan-Bernd Stukenborg

Related Concepts

Freshwater eels
Gene Transfer Techniques
cDNA Probes
Coculture Techniques
Green Fluorescent Proteins

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.