PMID: 3762560Sep 1, 1986Paper

Transfer of the E. coli O6-methylguanine methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N'-nitro-N-nitrosoguanidine

Mutation Research
K IshizakiM Ikenaga

Abstract

We have constructed a plasmid on which the E. coli O6-methylguanine-DNA methyltransferase (MT) gene (ada gene) was linked with an SV40 promoter sequence and a poly(A) site. After transferring this plasmid into Mer- HeLa MR cells by DNA transfection, effective expression of E. coli MT was observed. Isolated stable transformant clones showed higher resistance to N-methyl-N'-nitro-N-nitrosoguanidine in colony formation and sister-chromatid exchange induction than HeLa MR cells.

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Citations

Apr 12, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yasunari SakaiYusaku Nakabeppu
Nov 1, 1990·Mutation Research
Nov 1, 1990·Mutation Research·L den EngelseE Scherer
Jan 1, 1987·Pharmacology & Therapeutics·A E Pegg, M E Dolan
Jan 1, 1988·Pharmacology & Therapeutics·R B Painter
Feb 1, 1992·Cancer Genetics and Cytogenetics·B RümmeleinH W Rüdiger
Apr 18, 2000·Mutation Research·R B Roth, L D Samson
Apr 12, 2000·European Journal of Biochemistry·G Frosina
Jan 9, 2003·The Lancet Oncology·Mark R Middleton, Geoffrey P Margison
Jan 1, 1991·Radiation and Environmental Biophysics·B KainaT Coquerelle
Jun 9, 2005·Nucleic Acids Research·Elias B JacksonTadahide Izumi
Jan 6, 2018·Biomedicines·Ilya O VelegzhaninovOlesya M Vakhrusheva
Sep 1, 1990·Mutation Research·P Karran, C Stephenson
Nov 1, 1990·Mutation Research·V M MaherJ J McCormick

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