PMID: 8586262Dec 15, 1995Paper

Transfer of the IncJ plasmid R391 to recombination deficient Escherichia coli K12: evidence that R391 behaves as a conjugal transposon

FEMS Microbiology Letters
D B Murphy, J T Pembroke

Abstract

A study of the IncJ plasmid R391 confirmed a low frequency of transfer between recombination proficient (recA+) Escherichia coli (10(-5) donor -1). Reanalysis of its transfer to recombination deficient (recA) E. coli revealed an equivalent transfer frequency to and from all mutants tested. Extrachromosomal DNA could not be detected in either recA+ or recA transconjugants, while R391 proved refractory to curing in both backgrounds implying a high degree of stability. The integration of R391 into a specific region of the chromosome was demonstrated by its transfer as part of the exogenote mobilised from the transfer origins of Hfr strains BW6165 and JC158. Transfer of R391 coupled to recA independent chromosomal integration has significant implications as to the nature and classification of the element. We propose that R391 behaves like a conjugal transposon.

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Citations

Apr 27, 1999·Molecular Microbiology·B Hochhut, M K Waldor
Aug 24, 2002·Journal of Bacteriology·Dietmar BöltnerA Mark Osborn
Dec 1, 2005·Journal of Microbiological Methods·Barry M McGrathJ Tony Pembroke
Aug 20, 2005·Letters in Applied Microbiology·J T Pembroke, B M McGrath
Sep 13, 2012·Journal of Microbiological Methods·Bethany N KentMichael J Calcutt
Jul 7, 2011·FEMS Microbiology Reviews·Mark A Toleman, Timothy R Walsh
Dec 15, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Michael G NapolitanoE Fidelma Boyd
May 26, 1999·FEMS Microbiology Letters·D B Murphy, J T Pembroke
Dec 24, 2008·Animal Health Research Reviews·Patrick Boerlin, Richard J Reid-Smith
Feb 7, 2014·FEMS Microbiology Reviews·Patricia SiguierMick Chandler
Apr 1, 1997·Journal of Bacteriology·B HochhutK Schmid

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